Marine Agriculture Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101, China.
Agricultural College, Qingdao Agricultural University, Qingdao, 266109, China.
Carbohydr Polym. 2018 Feb 1;181:902-910. doi: 10.1016/j.carbpol.2017.11.061. Epub 2017 Nov 20.
Sulfated polysaccharides from green tide algae Ulva prolifera were effectively extracted by microwave assisted hydrothermal extraction technology. The properties and bioactivities of polysaccharides could be controlled by extraction conditions. While rhamnose, galacturonic acid and glucose were major monosaccharides at 90°C with 0.01M HCl, glucose was the major monomer at 150°C with 0.1M HCl. Sulfur content increased with temperature and acid concentration, while molecular weight decreased with temperature and concentration. Functional property analysis showed that polysaccharides extracted from 90°C, 0.05M HCl had the best water-holding capacity (41.32g/g) and oil-holding capacity (15.09g/g), and polysaccharides extracted from 150°C, 0.05M HCl had the best foaming properties (foaming capacity 143% and foaming stability 113%, respectively). In addition, polysaccharides extracted from 150°C, 0.1M HCl exhibited higher antioxidant activity and pancreatic lipase inhibition activity. The relationship between polysaccharide compositions/molecular weight and properties/bioactivities was discussed.
绿潮藻类浒苔中的硫酸多糖可通过微波辅助水热提取技术有效地提取。多糖的性质和生物活性可以通过提取条件来控制。在 90°C 下用 0.01M HCl 提取时,主要单糖为鼠李糖、半乳糖醛酸和葡萄糖,而在 150°C 下用 0.1M HCl 提取时,主要单体为葡萄糖。随着温度和酸浓度的增加,硫含量增加,而分子量则随着温度和浓度的增加而降低。功能特性分析表明,在 90°C、0.05M HCl 下提取的多糖具有最佳的持水力(41.32g/g)和持油力(15.09g/g),而在 150°C、0.05M HCl 下提取的多糖具有最佳的泡沫性能(泡沫能力为 143%,泡沫稳定性为 113%)。此外,在 150°C、0.1M HCl 下提取的多糖具有更高的抗氧化活性和胰脂肪酶抑制活性。讨论了多糖组成/分子量与性质/生物活性之间的关系。