Department of Radiology, Seoul National University Hospital, Seoul, Korea.
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Korea.
Sci Rep. 2017 Dec 18;7(1):17740. doi: 10.1038/s41598-017-17062-1.
Isocitrate dehydrogenase 1 (IDH1)-wildtype glioblastoma (GBM) has found to be accompanied with increased expression of branched-chain amino acid trasaminase1 (BCAT1), which is associated with tumor growth and disease progression. In this retrospective study, quantitative RT-PCR, immunohistochemistry, and western blot were performed with GBM patient tissues to evaluate the BCAT1 level. Quantitative MR imaging parameters were evaluated from DSC perfusion imaging, DWI, contrast-enhanced T1WI and FLAIR imaging using a 3T MR scanner. The level of BCAT1 was significantly higher in IDH1-wildtype patients than in IDH1-mutant patients obtained in immunohistochemistry and western blot. The BCAT1 level was significantly correlated with the mean and 95 percentile-normalized CBV as well as the mean ADC based on FLAIR images. In addition, the 95 percentile-normalized CBV from CE T1WI also had a significant correlation with the BCAT1 level. Moreover, the median PFS in patients with BCAT1 expression <100 was longer than in those with BCAT1 expression ≥100. Taken together, we found that a high BCAT1 level is correlated with high CBV and a low ADC value as well as the poor prognosis of BCAT1 expression is related to the aggressive nature of GBM.
异柠檬酸脱氢酶 1（IDH1）-野生型脑胶质瘤（GBM）被发现伴随着支链氨基酸转氨酶 1（BCAT1）的表达增加，这与肿瘤生长和疾病进展有关。在这项回顾性研究中，使用 GBM 患者组织进行了定量 RT-PCR、免疫组织化学和 Western blot 检测，以评估 BCAT1 水平。使用 3T MR 扫描仪对 DSC 灌注成像、DWI、增强 T1WI 和 FLAIR 成像的定量 MR 成像参数进行了评估。在免疫组织化学和 Western blot 中，IDH1 野生型患者的 BCAT1 水平明显高于 IDH1 突变型患者。BCAT1 水平与基于 FLAIR 图像的平均和 95 百分位归一化 CBV 以及平均 ADC 显著相关。此外，CE T1WI 的 95 百分位归一化 CBV 与 BCAT1 水平也有显著相关性。此外，BCAT1 表达<100 的患者的中位 PFS 长于 BCAT1 表达≥100 的患者。综上所述，我们发现高 BCAT1 水平与高 CBV 和低 ADC 值相关，BCAT1 表达不良的预后与 GBM 的侵袭性有关。
