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埃塞俄比亚南部和西南部玉米籽粒的多种霉菌毒素及真菌分析

Multimycotoxin and fungal analysis of maize grains from south and southwestern Ethiopia.

作者信息

Getachew Alemayehu, Chala Alemayehu, Hofgaard Ingerd Skow, Brurberg May Bente, Sulyok Michael, Tronsmo Anne-Marte

机构信息

a College of Agriculture , Hawassa University , Hawassa , Ethiopia.

b Department of Plant Sciences , Norwegian University of Life Sciences , Ås , Norway.

出版信息

Food Addit Contam Part B Surveill. 2018 Mar;11(1):64-74. doi: 10.1080/19393210.2017.1408698. Epub 2017 Dec 19.

DOI:10.1080/19393210.2017.1408698
PMID:29258380
Abstract

The natural occurrence of fungi, mycotoxins and fungal metabolites was investigated in 100 samples of maize grains collected from south and southwestern Ethiopia in 2015. The maize samples were contaminated by Fusarium, Aspergillus and Penicillium species. Using liquid chromatography tandem mass spectrometry 127 secondary metabolites were analysed. Zearalenone was the most prevalent mycotoxin, occurring in about 96% of the samples. Zearalenone sulfate was the second most prevalent, present in 81% of the samples. Fumonisin B1 was detected in 70% of the samples with a mean level of 606 μg kg in positive samples, while FB2, FB3 and FB4 were detected in 62%, 51% and 60% of the maize samples with mean levels of 202, 136 and 85 μg kg, respectively. Up to 8% of the samples were contaminated with aflatoxins, with a maximum level of aflatoxin B1 of 513 μg kg. Results were higher than earlier reports for maize from Ethiopia.

摘要

2015年,对从埃塞俄比亚南部和西南部采集的100份玉米籽粒样本中真菌、霉菌毒素和真菌代谢产物的自然发生情况进行了调查。玉米样本受到镰刀菌属、曲霉属和青霉属物种的污染。使用液相色谱串联质谱法分析了127种次生代谢产物。玉米赤霉烯酮是最普遍存在的霉菌毒素,约96%的样本中存在该毒素。硫酸玉米赤霉烯酮是第二普遍存在的,81%的样本中含有该毒素。70%的样本中检测到伏马毒素B1,阳性样本中的平均含量为606μg/kg,而在62%、51%和60%的玉米样本中分别检测到FB2、FB3和FB4,平均含量分别为202μg/kg、136μg/kg和85μg/kg。高达8%的样本被黄曲霉毒素污染,黄曲霉毒素B1的最高含量为513μg/kg。结果高于埃塞俄比亚此前关于玉米的报告。

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