重组酶聚合酶扩增联合侧向流层析检测 。
Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of .
机构信息
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Sarawak State Health Department, Kuching, Sarawak, Malaysia.
出版信息
Am J Trop Med Hyg. 2018 Mar;98(3):700-703. doi: 10.4269/ajtmh.17-0738. Epub 2017 Dec 14.
Abstract
The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of . With incubation at 37°C, the gene of was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of . Nonetheless, it was demonstrated that all ( = 41) and other sp. ( = 25) were positive while negative samples ( = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings.
摘要
本研究旨在开发一种重组酶聚合酶扩增（RPA）与侧向流动（LF）条带方法相结合，用于 的特异性诊断。在 37°C 孵育下， 基因在 12 分钟内成功扩增。通过在反应溶液中添加特异性设计的探针，可以在 LF 条带中可视化扩增的 RPA 产物。RPA 检测法具有较高的灵敏度，检测下限低至 10 个寄生虫/μL 的 。然而，结果表明所有 （=41）和其他 sp.（=25）均为阳性，而阴性样本（=8）均为阴性。因此，RPA 和 LF 条带检测的组合是一种很有前途的方法，有可能适用于资源有限的环境。
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