Tehran University of Medical Sciences, Department of Microbiology, School of Medicine, Tehran, Iran.
Private practice, Tehran, Iran.
Braz Oral Res. 2017 Dec 18;31:e108. doi: 10.1590/1807-3107bor-2017.vol31.0108.
Endodontic infections are considered to be caused by the presence of various microorganisms within the root canal system. Recognition of this microbiota contributes to the successful treatment of infected root canals. This study investigated the microorganisms associated with primary and secondary endodontic infections via culture methods, biochemical tests, and molecular approaches in an Iranian population. Microbial specimens were collected from 36 patients with primary endodontic infection and 14 patients with a history of root canal therapy. Advanced microbiological culture techniques were used to isolate microbiota; subsequently, biochemical tests and 16S ribosomal RNA gene sequencing were performed to identify the microorganisms. Within the total 218 cultivable isolates, Veillonella parvula (20.6%) was found to occur with the highest frequency in primary endodontic infection, followed by Porphyromonas gingivalis (14.1%), and Aggregatibacter actinomycetemcomitans (9.2%). Enterococcus faecalis (36.6%) was the most predominant microorganism in secondary endodontic infections, followed by Candida albicans, Propionibacterium acnes, and V. parvula with frequencies of 20%, 2%, and 2%, respectively. It was concluded that V. parvula and E. faecalis was most frequently found in primary and secondary endodontic infections, respectively.
牙髓感染被认为是由根管系统内各种微生物的存在引起的。识别这种微生物群有助于成功治疗感染的根管。本研究通过培养方法、生化试验和分子方法，在伊朗人群中调查了与原发性和继发性牙髓感染相关的微生物。从 36 例原发性牙髓感染患者和 14 例根管治疗史患者中采集微生物标本。采用先进的微生物培养技术分离菌群；随后进行生化试验和 16S 核糖体 RNA 基因测序鉴定微生物。在总共 218 株可培养分离株中，发现小韦荣球菌（20.6%）在原发性牙髓感染中发生频率最高，其次是牙龈卟啉单胞菌（14.1%）和伴放线放线杆菌（9.2%）。粪肠球菌（36.6%）是继发性牙髓感染中最主要的微生物，其次是白色念珠菌、痤疮丙酸杆菌和小韦荣球菌，分别占 20%、2%和 2%。结论：小韦荣球菌和粪肠球菌分别是原发性和继发性牙髓感染中最常发现的微生物。
