Department of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea.
National Center for Creative Research Initiatives, Seoul National University, Seoul, 08826, Republic of Korea.
Mol Brain. 2017 Dec 28;10(1):63. doi: 10.1186/s13041-017-0344-5.
Super-resolution fluorescence microscopy in the current form is hard to be used to image the neural connectivity of thick tissue samples due to problems such as slow imaging speed, severe photobleaching of fluorescent probes, and high background noise. Recently developed DNA-PAINT solved the photobleaching problem, but its imaging speed is extremely low. We report accelerated super-resolution fluorescence microscopy named FRET-PAINT. Compared to conventional DNA-PAINT, the imaging speed of the microscopy increases up to ~30-fold. As demonstrations, we show that 25-50 second imaging time is long enough to provide super-resolution reconstruction of microtubules and mitochondria of COS-7 cells.
目前，由于成像速度慢、荧光探针严重光漂白以及背景噪声高等问题，传统的超分辨率荧光显微镜技术很难用于厚组织样品的神经连接成像。最近开发的 DNA-PAINT 技术解决了光漂白问题，但成像速度极慢。我们报告了一种名为 FRET-PAINT 的加速超分辨率荧光显微镜技术。与传统的 DNA-PAINT 相比，该显微镜的成像速度提高了约 30 倍。作为演示，我们展示了 25-50 秒的成像时间足以提供 COS-7 细胞的微管和线粒体的超分辨率重构。
