Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Science. 2014 Apr 4;344(6179):65-9. doi: 10.1126/science.1250944. Epub 2014 Mar 13.
DNA self-assembly has produced diverse synthetic three-dimensional polyhedra. These structures typically have a molecular weight no greater than 5 megadaltons. We report a simple, general strategy for one-step self-assembly of wireframe DNA polyhedra that are more massive than most previous structures. A stiff three-arm-junction DNA origami tile motif with precisely controlled angles and arm lengths was used for hierarchical assembly of polyhedra. We experimentally constructed a tetrahedron (20 megadaltons), a triangular prism (30 megadaltons), a cube (40 megadaltons), a pentagonal prism (50 megadaltons), and a hexagonal prism (60 megadaltons) with edge widths of 100 nanometers. The structures were visualized by means of transmission electron microscopy and three-dimensional DNA-PAINT super-resolution fluorescent microscopy of single molecules in solution.
DNA 自组装产生了多种合成的三维多面体。这些结构的分子量通常不超过 500 万道尔顿。我们报告了一种简单、通用的一步法自组装线框 DNA 多面体的策略，这种方法组装的多面体比以前大多数结构都要庞大。一个具有精确控制的角度和臂长的刚性三臂连接 DNA 折纸嵌段图案用于多面体的分级组装。我们通过实验构建了一个四面体（2000 万道尔顿）、一个三角棱柱（3000 万道尔顿）、一个立方体（4000 万道尔顿）、一个五棱柱（5000 万道尔顿）和一个六棱柱（6000 万道尔顿），其边缘宽度为 100 纳米。通过透射电子显微镜和溶液中单分子的三维 DNA-PAINT 超分辨率荧光显微镜观察到了这些结构。
