College of Food Science and Engineering, Northwest A&F University, 712100 Yangling, PR China.
Food Chem. 2018 Apr 15;245:1124-1130. doi: 10.1016/j.foodchem.2017.11.058. Epub 2017 Nov 16.
A neutral polysaccharide (PZMP1) with a molecular weight of 16.97 kDa was isolated from Ziziphus Jujuba cv. Muzao with water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. PZMP1 was structurally characterized with high-performance gel-permeation chromatography, gas chromatography, Fourier transform infrared spectroscopy, methylation analysis, NMR spectroscopy, X-ray diffraction, scanning electron microscopy, and atomic force microscopy. The results show that PZMP1 is a homogeneous heteropolysaccharide composed of arabinose, galactose, glucose, mannose, and xylose in a molar percentage of 17.36: 3.29:2.68:1.05:1.00. The majorly backbone of PZMP1 is composed of 1,3,5-linked Araf, 1,3-linked Araf, 1,5-linked Araf, 1,4-linked Glcp, and 1-linked Araf, 1-linked Glcp. According to an NMR analysis, the repeating unit of PZMP1 is a linear backbone with branches at positions O-3, and O-5. PZMP1 also displays effective dose-dependent hypolipidemic activity, suggesting that it is a natural hypolipidemic agent, which could contribute to the functional food qualities of jujube fruit.
从大枣品种木枣中用水提醇沉和 DEAE-Sepharose Fast Flow 和 Sephacryl S-300 柱层析等方法分离得到一种中性多糖（PZMP1），其分子量为 16.97 kDa。采用高效凝胶渗透色谱、气相色谱、傅里叶变换红外光谱、甲基化分析、NMR 光谱、X 射线衍射、扫描电子显微镜和原子力显微镜等方法对 PZMP1 的结构进行了表征。结果表明，PZMP1 是一种均相杂多糖，由阿拉伯糖、半乳糖、葡萄糖、甘露糖和木糖组成，摩尔百分比为 17.36:3.29:2.68:1.05:1.00。PZMP1 的主要骨架由 1,3,5-连接的阿拉伯呋喃糖基（Araf）、1,3-连接的阿拉伯呋喃糖基、1,5-连接的阿拉伯呋喃糖基、1,4-连接的葡萄糖基（Glcp）和 1-连接的阿拉伯呋喃糖基、1-连接的葡萄糖基组成。根据 NMR 分析，PZMP1 的重复单元是一条带有 O-3 和 O-5 位置支链的线性主链。PZMP1 还表现出有效剂量依赖性的降血脂活性，表明它是一种天然的降血脂剂，这可能有助于枣果的功能性食品品质。
