Qiu Xinyun, Ma Jingjing, Jiao Chunhua, Mao Xiaqiong, Zhao Xiaojing, Lu Meijiao, Wang Kai, Zhang Hongjie
Department of Gastroenterology, First Affliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Oncotarget. 2017 Nov 20;8(64):107577-107588. doi: 10.18632/oncotarget.22534. eCollection 2017 Dec 8.
Fungi colonize the human gut and might play a key role in the pathogenesis of ulcerative colitis (UC). However, studies on the fungal composition in the gut (especially adhering to the intestinal mucosa) of UC patients is limited.
The number of fungi decreased significantly in inflamed mucosa compared with that in HS mucosa. Fifteen major genera were examined, among which , unidentified genus of , , , and showed increasing trends, whereas , , , , , , and showed decreasing trends in UC patients compared to the HS. The pro-inflammatory cytokines (IL-Iβ, TNF-α, INF-γ, IL-6, IL-17A, and IL-23) were up-regulated in the UC group. The genera , , and were positively correlated, while and were negatively correlated with the expression of several colonic pro-inflammatory cytokines and the Baron and/or Mayo score.
Our study confirms the alteration of the colonic fungal microbiota in the UC patients, which might be associated with mucosal inflammation and pathogenesis of UC. Further studies need to identify the roles of different intestinal fungi in detail, and to determine the mechanism of the host-fungal interaction underlying the development of UC.
Mucosal samples of inflamed descending colon from 14 active UC patients and 15 healthy subjects (HS) were analyzed by high-throughput sequencing to compare the fungal microbiota. The expressions of pro-inflammatory cytokines (IL-Iβ, TNF-α, INF-γ, IL-6, IL-17A, and IL-23) in intestinal mucosal tissues were examined. The Baron and Mayo scores of UC patients were evaluated, and the correlation between intestinal fungal composition and intestinal inflammatory status was analyzed.
真菌定殖于人体肠道，可能在溃疡性结肠炎（UC）的发病机制中起关键作用。然而，关于UC患者肠道（尤其是附着于肠黏膜的真菌）真菌组成的研究有限。
与健康对照（HS）黏膜相比，炎症黏膜中的真菌数量显著减少。检测了15个主要菌属，其中未鉴定的属、属、属和属在UC患者中呈增加趋势，而属、属、属、属、属、属和属与HS相比呈减少趋势。UC组促炎细胞因子（IL-1β、TNF-α、INF-γ、IL-6、IL-17A和IL-23）上调。属、属和属与几种结肠促炎细胞因子的表达以及Baron和/或Mayo评分呈正相关，而属和属呈负相关。
我们的研究证实了UC患者结肠真菌微生物群的改变，这可能与黏膜炎症和UC的发病机制有关。需要进一步研究详细确定不同肠道真菌的作用，并确定UC发生发展过程中宿主-真菌相互作用的机制。
通过高通量测序分析14例活动期UC患者和15例健康对照（HS）降结肠炎症黏膜样本，以比较真菌微生物群。检测肠黏膜组织中促炎细胞因子（IL-1β、TNF-α、INF-γ、IL-6、IL-17A和IL-23）的表达。评估UC患者的Baron和Mayo评分，并分析肠道真菌组成与肠道炎症状态之间的相关性。
