Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, Pennsylvania.
University of Virginia Center for Research in Reproduction, Charlottesvile, Virginia.
Fertil Steril. 2018 Jan;109(1):137-141.e2. doi: 10.1016/j.fertnstert.2017.09.029.
To validate the ability of serum kisspeptin-54 to discriminate between first-trimester viable pregnancies and miscarriages.
Case-control study.
Academic medical centers.
PATIENT(S): Women with confirmed viable intrauterine pregnancy (IUP) at estimated gestational age 6-10 weeks (n = 20), women with confirmed miscarriage (spontaneous abortion [SAB]) at estimated gestational age 6-10 weeks (n = 20), and nonpregnant women (n = 19).
INTERVENTION(S): Collection of serum samples from women with confirmed IUP, SAB, and nonpregnant women for the measurement of serum kisspeptin and serum hCG levels.
MAIN OUTCOME MEASURE(S): Serum kisspeptin and hCG.
RESULT(S): The limit of detection was 0.024 ng/mL; intra- and interassay coefficients of variation were 5.1% and 8.6%, respectively. Kisspeptin levels differed between the pregnant and nonpregnant state and by viability. Kisspeptin levels were positively associated with gestational age. There was also a significant positive association with hCG in SAB, but not in IUP.
CONCLUSION(S): Plasma levels of kisspeptin have been suggested as a biomarker for miscarriage. This study demonstrates kisspeptin assay stability in serum and its potential clinical utility as a biomarker for early pregnancy viability.
验证血清 kisspeptin-54 区分早孕期存活妊娠与流产的能力。
病例对照研究。
学术医疗中心。
经估计孕龄 6-10 周证实为宫内活胎（IUP）的女性（n=20）、经估计孕龄 6-10 周证实为流产（自然流产[SAB]）的女性（n=20）和未怀孕的女性（n=19）。
采集经估计孕龄 6-10 周证实为 IUP、SAB 和未怀孕女性的血清样本，用于检测血清 kisspeptin 和 hCG 水平。
血清 kisspeptin 和 hCG。
检测下限为 0.024ng/mL；批内和批间变异系数分别为 5.1%和 8.6%。妊娠和非妊娠状态以及存活情况影响 kisspeptin 水平。kisspeptin 水平与孕龄呈正相关。在 SAB 中，hCG 与 kisspeptin 也呈显著正相关，但在 IUP 中则无此相关性。
血浆 kisspeptin 水平已被认为是流产的生物标志物。本研究表明，血清 kisspeptin 测定具有稳定性，可能具有作为早期妊娠存活的生物标志物的临床应用价值。
