Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh , Crewe Road South, Edinburgh EH4 2XR, United Kingdom.
MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh , Edinburgh EH16 4TJ, United Kingdom.
ACS Appl Mater Interfaces. 2018 Jan 31;10(4):3341-3348. doi: 10.1021/acsami.7b17282. Epub 2018 Jan 22.
Herein, we report a straightforward method for the scalable preparation of Pd nanoparticles (Pd-NPs) with reduced inherent cytotoxicity and high photothermal conversion capacity. These Pd-NPs are rapidly taken up by cells and able to kill labeled cancer cells upon short exposure to near-infrared (NIR) light. Following cell treatment with Pd-NPs, ablated areas were patterned with high precision by laser scanning microscopy, allowing one to perform cell migration assays with unprecedented accuracy. Using coherent Raman microscopy, cells containing Pd-NPs were simultaneously ablated and imaged. This novel methodology was combined with intravital imaging to mediate microablation of cancerous tissue in tumor xenografts in mice.
在此，我们报告了一种简单的方法，可以大规模制备具有降低固有细胞毒性和高光热转换能力的钯纳米粒子（Pd-NPs）。这些 Pd-NPs 被细胞迅速摄取，并在短时间内暴露于近红外（NIR）光下能够杀死标记的癌细胞。用 Pd-NPs 处理细胞后，用光扫描显微镜以高精度对消融区域进行图案化，从而可以以前所未有的精度进行细胞迁移实验。使用相干拉曼显微镜，同时对含有 Pd-NPs 的细胞进行消融和成像。该新方法与活体成像相结合，用于介导小鼠肿瘤异种移植中癌变组织的微消融。
