Ratnapuram Hari Prasad, Vutukuru S S, Yadavalli Rajasri
Department of Biotechnology, Sreenidhi Institute of Science and Technology (Autonomous), Yamnampet, Ghatkesar, Hyderabad, Telangana, India.
Heliyon. 2018 Jan 4;4(1):e00496. doi: 10.1016/j.heliyon.2017.e00496. eCollection 2018 Jan.
Influence of mixotrophic mode and its transition to various trophic modes under stress conditions was assessed during two stage cultivation of . Significant lipid productivity was triggered under low light intensity, glucose + bicarbonate supplementation and nitrogen starvation. The association between biomass and lipid productivity, fatty acid composition during mixotrophic transition was critically evaluated. Biomass in growth phase (GP) and stress phase (SP) was 6.14 g/l and 5.14 g/l, respectively, in mixotrophic mode. Higher lipid productivity of 284 g/kg and 154.3 g/kg of neutral lipids was achieved in SP in mixotrophic-mixotrophic (MM) and mixotrophic-heterotrophic (MH) modes, respectively. Stress conditions resulted in high unsaturated fatty acid methyl esters in MH mode. In addition, neutral lipid content was 58% in MH and 52% in MM, that can be attributed to carbon source that is supplemented even in stress phase. Exploring such novel strategies can generate sustainable avenues for biodiesel production.
在两个阶段的培养过程中，评估了混合营养模式及其在胁迫条件下向各种营养模式转变的影响。在低光照强度、补充葡萄糖 + 碳酸氢盐以及氮饥饿的条件下，引发了显著的脂质生产力。对混合营养转变过程中生物量与脂质生产力、脂肪酸组成之间的关联进行了严格评估。在混合营养模式下，生长阶段（GP）和胁迫阶段（SP）的生物量分别为6.14 g/L和5.14 g/L。在混合营养 - 混合营养（MM）模式和混合营养 - 异养（MH）模式的胁迫阶段，分别实现了284 g/kg和154.3 g/kg的较高中性脂质脂质生产力。胁迫条件导致MH模式下的不饱和脂肪酸甲酯含量较高。此外，MH模式下中性脂质含量为58%，MM模式下为52%，这可归因于即使在胁迫阶段也补充的碳源。探索此类新策略可为生物柴油生产开辟可持续途径。
