对亨廷顿舞蹈症患者尸检脑样本的重组DNA研究。
Recombinant DNA studies on stored necropsy brain samples from patients with Huntington's chorea.
作者信息
Upadhyaya M, Reynolds G P, Harper P S
出版信息
J Clin Pathol. 1985 Oct;38(10):1093-5. doi: 10.1136/jcp.38.10.1093.
An analysis of deoxyribonucleic acid (DNA) in deep frozen brain samples taken from 100 patients with Huntington's chorea after death showed undegraded DNA in 44 cases. Of these, 16 were analysed with G8, a recombinant DNA probe, linked to the Huntington's chorea locus. In all cases unambiguous Southern blots were obtainable. No correlation between the yield of DNA and the principal storage factors was observed. The use of stored brain tissue obtained after death from patients with Huntington's chorea should be considered when analysis of DNA is needed for predictive studies, but DNA should preferably be isolated from the tissue before storage as degradation in these samples can occur.
对100例亨廷顿舞蹈症患者死后提取的深度冷冻脑样本中的脱氧核糖核酸（DNA）进行分析，结果显示44例样本中的DNA未降解。其中16例样本用与亨廷顿舞蹈症基因座相连的重组DNA探针G8进行了分析。在所有病例中均可获得清晰明确的Southern印迹。未观察到DNA产量与主要储存因素之间存在相关性。当需要进行预测性研究而分析DNA时，应考虑使用亨廷顿舞蹈症患者死后获得的储存脑组织，但最好在储存前从组织中分离出DNA，因为这些样本可能会发生降解。
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