Seura Takahiro, Yoshino Yoko, Fukuwatari Tsutomu
Department of Sports and Health Sciences, Faculty of Health and Medical Sciences, Aichi Shukutoku University.
Department of Nutrition, School of Human Cultures, The University of Shiga Prefecture.
J Nutr Sci Vitaminol (Tokyo). 2017;63(6):396-404. doi: 10.3177/jnsv.63.396.
Recent studies have shown that dietary content affects the health of the host by changing the gut microbiota. However, little is known about the association of microbiota composition with habitual diet in Japanese people. Here, we aimed to clarify the relationship between the fecal microbiota and habitual dietary intake of micronutrients, macronutrients and food groups in healthy young Japanese women. Analysis of fecal microbiota was performed by the terminal restriction fragment length polymorphism (T-RFLP) method, and a dietary survey was conducted over three consecutive days using a weighed food record method. T-RFLP pattern analysis divided the subjects into two clusters, where cluster A group had a high relative abundance of Bacteroides and Clostridium cluster IV, and cluster B group had a high relative abundance of Bifidobacterium and Lactobacillales. Cluster A group also had lower intakes of iron and vitamin K and higher intakes of mushrooms and snacks than cluster B group. Analysis of Spearman rank correlations found several significant relationships between fecal microbiota and intake of nutrients and food groups. Bifidobacterium was correlated with iron intake, and Clostridium cluster XI was negatively correlated with intakes of cholesterol and eggs. These results suggest that dietary habits may strongly affect Bifidobacterium, Bacteroides and Clostridium abundance in the gut microbiota of young Japanese women. This is the first study to show relationships between fecal microbiota and habitual dietary intake in Japanese people. Accumulation of results from similar studies will help to elucidate the relationships between dietary intake and diseases in Japanese people.
最近的研究表明，饮食成分通过改变肠道微生物群来影响宿主健康。然而，对于日本人肠道微生物群组成与日常饮食之间的关联却知之甚少。在此，我们旨在阐明健康年轻日本女性的粪便微生物群与微量营养素、常量营养素及食物组的日常饮食摄入之间的关系。采用末端限制性片段长度多态性（T-RFLP）方法对粪便微生物群进行分析，并使用称重食物记录法连续三天进行饮食调查。T-RFLP模式分析将受试者分为两个聚类，其中A聚类组中拟杆菌属和梭菌属第四簇的相对丰度较高，而B聚类组中双歧杆菌属和乳杆菌目的相对丰度较高。A聚类组中铁和维生素K的摄入量也低于B聚类组，而蘑菇和零食的摄入量则高于B聚类组。Spearman等级相关性分析发现粪便微生物群与营养素及食物组摄入量之间存在若干显著关系。双歧杆菌与铁摄入量相关，梭菌属第十一簇与胆固醇和鸡蛋的摄入量呈负相关。这些结果表明，饮食习惯可能会强烈影响年轻日本女性肠道微生物群中双歧杆菌、拟杆菌属和梭菌属的丰度。这是第一项揭示日本人粪便微生物群与日常饮食摄入之间关系的研究。类似研究结果的积累将有助于阐明日本人饮食摄入与疾病之间的关系。
