Department of Clinical Laboratory, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
Dis Markers. 2017;2017:7293459. doi: 10.1155/2017/7293459. Epub 2017 Dec 3.
It has been reported that several microRNAs (miRNAs), such as miR-141, miR-9, and miR-122, are involved in the regulation of pancreatitis-related proteins or that their levels change in acute pancreatitis (AP) animal models. However, the serum levels, as well as the clinical diagnostic and prognostic values, of these miRNAs in AP patients remain unclear. Furthermore, as a pancreas- (islet) enriched miRNA, miR-7 was reported to be downregulated in AP patients, which requires further verification.
The levels of miR-7, miR-9, miR-122, and miR-141 were examined and compared using qRT-PCR among 80 severe AP patients, 80 mild AP patients, and 74 healthy controls.
The serum levels of these four miRNAs were increased markedly in the AP patients compared with the controls, and these levels decreased significantly after effective therapy. Particularly, the level of miR-7 was higher in severe AP patients than in mild AP patients. ROC curve analysis demonstrated that four miRNAs could be used as potential biomarkers for AP. Moreover, these miRNAs showed strong positive correlations with CRP, which may be associated with inflammation.
The serum miR-7, miR-9, miR-122, and miR-141 levels were increased in AP patients. These 4 miRNAs may represent diagnostic and prognostic biomarkers for AP.
已有报道称，几种 microRNAs（miRNAs），如 miR-141、miR-9 和 miR-122，参与了胰腺炎相关蛋白的调控，或者它们在急性胰腺炎（AP）动物模型中的水平发生变化。然而，这些 miRNAs 在 AP 患者中的血清水平及其临床诊断和预后价值仍不清楚。此外，作为一种胰腺（胰岛）富集的 miRNA，miR-7 被报道在 AP 患者中下调，这需要进一步验证。
采用 qRT-PCR 法检测 80 例重症 AP 患者、80 例轻症 AP 患者和 74 例健康对照者血清中 miR-7、miR-9、miR-122 和 miR-141 的水平并进行比较。
与对照组相比，AP 患者这四种 miRNAs 的血清水平明显升高，经有效治疗后显著降低。特别是，重症 AP 患者的 miR-7 水平高于轻症 AP 患者。ROC 曲线分析表明，这四种 miRNAs 可作为 AP 的潜在生物标志物。此外，这些 miRNAs 与 CRP 呈强烈正相关，可能与炎症有关。
AP 患者血清 miR-7、miR-9、miR-122 和 miR-141 水平升高。这 4 种 miRNAs 可能代表 AP 的诊断和预后生物标志物。
