Capjak Ivona, Goreta Sandra Šupraha, Jurašin Darija Domazet, Vrček Ivana Vinković
1Croatian Institute of Transfusion Medicine, Petrova 3, 10000, Zagreb, Croatia.
2University of Zagreb, Faculty of Pharmacy and Biochemistry, Šalata ul. 2, 10000, Zagreb, Crotia.
Arh Hig Rada Toksikol. 2017 Dec 20;68(4):245-253. doi: 10.1515/aiht-2017-68-3054.
Nanomedicine is a booming medical field that utilises nanoparticles (NPs) for the development of medicines, medical devices, and diagnostic tools. The behaviour of NPs in vivo may be quite complex due to their interactions with biological molecules. These interactions in biological fluids result in NPs being enveloped by dynamic protein coronas, which serve as an interface between NPs and their environment (blood, cell, tissue). How will the corona interact with this environment will depend on the biological, chemical, and physical properties of NPs, the properties of the proteins that make the corona, as well as the biological environment. This review summarises the main characteristics of protein corona and describes its dynamic nature. It also presents the most common analytical methods to study the corona, including examples of protein corona composition for the most common NPs used in biomedicine. This knowledge is necessary to design NPs that will create a corona with a desired efficiency and safety in clinical use.
纳米医学是一个蓬勃发展的医学领域，它利用纳米颗粒（NPs）来开发药物、医疗设备和诊断工具。由于纳米颗粒与生物分子的相互作用，其在体内的行为可能相当复杂。这些在生物流体中的相互作用导致纳米颗粒被动态蛋白质冠层包裹，蛋白质冠层充当纳米颗粒与其环境（血液、细胞、组织）之间的界面。蛋白质冠层如何与这种环境相互作用将取决于纳米颗粒的生物学、化学和物理性质、构成蛋白质冠层的蛋白质性质以及生物环境。本综述总结了蛋白质冠层的主要特征并描述了其动态性质。它还介绍了研究蛋白质冠层的最常见分析方法，包括生物医学中最常用纳米颗粒的蛋白质冠层组成示例。这些知识对于设计在临床使用中能以所需效率和安全性形成蛋白质冠层的纳米颗粒是必要的。
