Voigt W H, Zeiler H J
Arzneimittelforschung. 1985;35(10):1600-3.
Killing curves were performed in pooled active human serum using serum resistant Escherichia coli C14, Pseudomonas aeruginosa 220 and Staphylococcus aureus 25151 as test organisms. Ciprofloxacin (a quinoline-carboxylic acid derivative, Bay o 9867) was added in concentrations of 0.025-4.0 micrograms/ml. Samples were taken at different times to determine the number of viable bacteria and to prepare specimens for transmission electron microscopy. The following drug concentration-dependent results were obtained: In the early phase of the bactericidal process in Escherichia coli C14 distinct loosenings of the peripheral cytoplasm occurred. With Pseudomonas aeruginosa 220 osmiophilic condensations were detected in the less dense areas where the bacterial DNA may be located. At low concentrations, elongation of gram-negative bacterial cells was observed, whereas at higher concentrations rupture and lysis occurred. In staphylococci, severe disturbances of septum formation and surface deformations could be found; in the final stage proteolysis and empty envelopes were observed.
使用血清耐受型大肠杆菌C14、铜绿假单胞菌220和金黄色葡萄球菌25151作为测试微生物，在混合的活性人血清中进行了杀菌曲线实验。加入浓度为0.025 - 4.0微克/毫升的环丙沙星（一种喹啉羧酸衍生物，拜耳o 9867）。在不同时间取样以确定活菌数量，并制备用于透射电子显微镜检查的标本。获得了以下药物浓度依赖性结果：在大肠杆菌C14杀菌过程的早期阶段，外周细胞质出现明显的疏松。对于铜绿假单胞菌220，在细菌DNA可能所在的密度较低区域检测到嗜锇凝聚物。在低浓度下，观察到革兰氏阴性细菌细胞伸长，而在高浓度下则发生破裂和裂解。在葡萄球菌中，可以发现隔膜形成的严重紊乱和表面变形；在最后阶段观察到蛋白水解和空包膜。
