Khan Ajmal, Naz Sadia, Farooq Umar, Shahid Muhammad, Ullah Irfan, Ali Iftikhar, Rauf Abdur, Mabkhot Yahia Nasser
Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
University of Nizwa Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, Oman.
J Pain Res. 2017 Dec 28;11:95-102. doi: 10.2147/JPR.S145551. eCollection 2018.
L. has been widely studied for its beneficial effect in inflammatory and pain conditions. The present study describes the isolation of two new bioactive chromone constituents from and their in vivo evaluation for anti-inflammatory and antinociceptive activities.
Two new chromone derivatives, namely, methyl 3-(2-(5-hydroxy-6-methoxy-4-oxo-4H-chromen-2-yl)ethyl)benzoate and 3-(1-hydroxy-2-(5-hydroxy-6-methoxy-4-oxo-4H-chromen-2-yl)ethyl)benzoic acid were isolated from and their structures were determined through various spectroscopic techniques including mass spectrometry, UV, IR, 1H NMR, 13C NMR, and two-dimensional-NMR like correlation spectroscopy and heteronuclear multiple bond correlation techniques. The isolated compounds (-) were tested for their prospective antinociceptive activity in acetic acid-induced abdominal constriction assay and anti-inflammatory activity in the carrageenan-induced paw edema assay in mice.
Significant attenuation (<0.001) of tonic visceral nociception was demonstrated by compound and at doses of 50 and 100 mg/kg. At similar doses, these compounds (-) also showed potent amelioration (<0.001) of carrageenan-induced paw swelling.
The isolated chromone derivatives (-) from are able to alleviate nociception and inflammation and the findings corroborated that may be used as a potential source of antinociceptive and anti-inflammatory candidates.
L. 因其在炎症和疼痛病症中的有益作用而受到广泛研究。本研究描述了从 中分离出两种新的具有生物活性的色酮成分，并对其抗炎和抗伤害感受活性进行了体内评估。
从 中分离出两种新的色酮衍生物，即3-(2-(5-羟基-6-甲氧基-4-氧代-4H-色烯-2-基)乙基)苯甲酸甲酯 和3-(1-羟基-2-(5-羟基-6-甲氧基-4-氧代-4H-色烯-2-基)乙基)苯甲酸 ，并通过各种光谱技术确定其结构，包括质谱、紫外光谱、红外光谱、1H核磁共振、13C核磁共振以及二维核磁共振，如相关光谱和异核多键相关技术。在小鼠的醋酸诱导腹部收缩试验中测试分离出的化合物(-)的潜在抗伤害感受活性，并在角叉菜胶诱导的爪肿胀试验中测试其抗炎活性。
化合物 和 在50和100 mg/kg剂量下显示出对强直性内脏伤害感受的显著减轻(<0.001)。在相似剂量下，这些化合物(-)对角叉菜胶诱导的爪肿胀也显示出有效改善(<0.001)。
从 中分离出的色酮衍生物(-)能够减轻伤害感受和炎症，研究结果证实 可能是抗伤害感受和抗炎候选物的潜在来源。
