Chattopadhyay Pronobesh, Hazarika Soilyadhar, Dhiman Sunil, Upadhyay Aadesh, Pandey Anurag, Karmakar Sanjeev, Singh Lokendra
Divisions of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam, India.
Pharmacognosy Res. 2012 Jul;4(3):134-7. doi: 10.4103/0974-8490.99072.
Vitex negundo L. (Verbenaceae) is a hardy plant widely distributed in the Indian subcontinent and used for treatment of a wide spectrum of health disorders in traditional and folk medicine, some of which have been experimentally validated. In present study, we aimed to investigate the anti-inflammatory effects of V. negundo in carrageenan-induced paw edema in rats, and to investigate the probable mechanism of anti-inflammatory action.
Paw edema was produced by injecting 1% solution of carrageenan, and the paw volume was measured before and after carrageenan injection up to 5 h. V. negundo leaf oil was extracted using a Clevenger apparatus and administered by a trans-dermal route to Wistar rats and the percentage of inhibition of inflammation was observed using a Plethysmometer by comparing a compound aerosol-based formulation with 1 mg diclofinac diethylamine BP and 7 mg methyl salicylate IP/kg body weight served as a standard drug whereas paraffin oil served as the placebo group. After withdrawing of blood, serum was separated and cyclooxygenase (COX)-1 and COX-2 inhibitory activities were measured by the enzyme immuno assay (EIA) method by using a COX inhibitor screening assay kit.
V. negundo leaf oil significantly (P < 0.05) reduced the carrageenan-induced paw edema as compared to the placebo group (paraffin oil) and 1 mg diclofinac diethylamine BP and 7 mg methyl salicylate IP showed the maximum inhibition of paw edema as compared to the V. negundo leaf oil treated group and the control group. Also in the present study V. negundo leaf oil showed significantly (P < 0.05) inhibits COX-1 pathways rather than COX-2 pathways as compared to the V. negundo leaf oil treated group.
It is suggested that the V. negundo leaf oil is a potent anti-inflammatory agent and acts via inhibition of COX-2 without much interfering COX-1 pathways.
黄荆（马鞭草科）是一种适应性强的植物，广泛分布于印度次大陆，在传统医学和民间医学中用于治疗多种健康疾病，其中一些已通过实验验证。在本研究中，我们旨在研究黄荆对大鼠角叉菜胶诱导的爪肿胀的抗炎作用，并探讨其抗炎作用的可能机制。
通过注射1%角叉菜胶溶液产生爪肿胀，并在注射角叉菜胶前后直至5小时测量爪体积。使用克莱文杰仪器提取黄荆叶油，并经皮给予Wistar大鼠，通过体积描记法观察炎症抑制百分比，将基于复方气雾剂的制剂与1mg双氯芬酸二乙胺BP和7mg水杨酸甲酯IP/kg体重作为标准药物进行比较，而石蜡油作为安慰剂组。采血后，分离血清，使用COX抑制剂筛选检测试剂盒通过酶免疫分析（EIA）方法测量环氧合酶（COX）-1和COX-2抑制活性。
与安慰剂组（石蜡油）相比，黄荆叶油显著（P<0.05）减轻了角叉菜胶诱导的爪肿胀，与黄荆叶油处理组和对照组相比，1mg双氯芬酸二乙胺BP和7mg水杨酸甲酯IP对爪肿胀的抑制作用最大。此外，在本研究中，与黄荆叶油处理组相比，黄荆叶油显著（P<0.
