Yan Zhaokun, Feng Jianyu, Peng Jun, Lai Zijun, Zhang Ling, Jin Yiyi, Yang Hong, Chen Wujin, Lin Jiumao
Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China.
Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China.
Oncol Lett. 2017 Dec;14(6):7923-7930. doi: 10.3892/ol.2017.7245. Epub 2017 Oct 20.
Willd (HDW) is a widely used traditional Chinese medicine in clinical therapy to treat various types of cancer, including colorectal cancer (CRC), but its effective polar fractions and functional mechanisms remain unclear. The aim of the present study was to determine the most effective extract of HDW and to investigate its effects on the regulation of CRC cell proliferation and apoptosis, as well as to investigate the underlying molecular mechanisms. The results demonstrated that the chloroform extract of HDW (CEHDW) exhibited the most anticancer ability. Furthermore, results of the MTT assay, colony formation, carboxyfluorescein diacetate succinimidyl ester assay and annexin V/propidium iodide staining suggested that CEHDW significantly inhibits proliferation and promotes apoptosis in the SW620 CRC cell line. Additionally, reverse transcription-polymerase chain reaction and western blot analysis demonstrated that CEHDW treatment downregulated the expression of Survivin, proliferating cell nuclear antigen, Cyclin D1, cyclin-dependent kinase 4 and B-cell lymphoma 2 (Bcl-2), and upregulated the expression of Bcl-2-associated X protein at the mRNA and protein levels. CEHDW also decreased the phosphorylation of protein kinase B (AKT) and extracellular-signal-regulated kinase (ERK), which indicated that the suppression of the AKT and ERK signaling pathways may be one of the underlying molecular mechanisms by which CEHDW exhibited its anticancer effect. Thus, CEHDW may be a promising agent for anticancer therapy.
威灵（HDW）是临床治疗中广泛使用的一种中药，用于治疗包括结直肠癌（CRC）在内的各种癌症，但其有效的极性组分和作用机制仍不清楚。本研究的目的是确定HDW最有效的提取物，研究其对CRC细胞增殖和凋亡调控的影响，并探讨潜在的分子机制。结果表明，HDW的氯仿提取物（CEHDW）具有最强的抗癌能力。此外，MTT法、集落形成实验、羧基荧光素二乙酸琥珀酰亚胺酯实验和膜联蛋白V/碘化丙啶染色结果表明，CEHDW显著抑制SW620 CRC细胞系的增殖并促进其凋亡。此外，逆转录-聚合酶链反应和蛋白质印迹分析表明，CEHDW处理在mRNA和蛋白质水平上下调了生存素、增殖细胞核抗原、细胞周期蛋白D1、细胞周期蛋白依赖性激酶4和B细胞淋巴瘤2（Bcl-2）的表达，并上调了Bcl-2相关X蛋白的表达。CEHDW还降低了蛋白激酶B（AKT）和细胞外信号调节激酶（ERK）的磷酸化，这表明抑制AKT和ERK信号通路可能是CEHDW发挥抗癌作用的潜在分子机制之一。因此，CEHDW可能是一种有前途的抗癌治疗药物。
