Pham Alan Chuong Q, Hei Erik La, Harvey John G, Holland Andrew Ja
The Children's Hospital at Westmead Burns Research Institute, The Burns Unit and The NSW Severe Burns Injury Service, The Children's Hospital at Westmead, The University of SydneyWestmead, NSW 2145, Australia.
Department of Paediatric Surgery, The Children's Hospital at Westmead, The University of SydneyWestmead, NSW 2145, Australia.
Int J Burns Trauma. 2017 Dec 20;7(7):147-152. eCollection 2017.
The aim of this study was to investigate the potential confounding effects of four different types of ambient lighting on the results of Laser Doppler Imaging (LDI) of a standardized cutaneous injury model.
After applying a mechanical stimulus to the anterior forearm of a healthy volunteer and inducing a wheal and arteriolar flare (the Triple response), we used a Laser Doppler Line Scanner (LDLS) to image the forearm under four different types of ambient lighting: light-emitting-diode (LED), compact fluorescent lighting (CFL), halogen, daylight, and darkness as a control. A spectrometer was used to measure the intensity of light energy at 785 nm, the wavelength used by the scanner for measurement under each type of ambient lighting.
Neither the LED nor CFL bulbs emitted detectable light energy at a wavelength of 785 nm. The color-based representation of arbitrary perfusion unit (APU) values of the Triple response measured by the scanner was similar between darkness, LED, and CFL light. Daylight emitted 2 mW at 785 nm, with a slight variation tending more towards lower APU values compared to darkness. Halogen lighting emitted 6 mW of light energy at 785 nm rendering the color-based representation impossible to interpret.
Halogen lighting and daylight have the potential to confound results of LDI of cutaneous injuries whereas LED and CFL lighting did not. Any potential sources of daylight should be reduced and halogen lighting completely covered or turned off prior to wound imaging.
本研究旨在调查四种不同类型的环境光对标准化皮肤损伤模型激光多普勒成像（LDI）结果的潜在混杂效应。
对一名健康志愿者的前臂施加机械刺激，诱发风团和小动脉潮红（三联反应）后，我们使用激光多普勒线扫描仪（LDLS）在四种不同类型的环境光下对前臂进行成像：发光二极管（LED）、紧凑型荧光灯（CFL）、卤素灯、日光，以及作为对照的黑暗环境。使用光谱仪测量在785nm处的光能强度，这是扫描仪在每种环境光类型下进行测量所使用的波长。
LED和CFL灯泡在785nm波长处均未发射出可检测到的光能。扫描仪测量的三联反应任意灌注单位（APU）值的基于颜色的表示在黑暗、LED和CFL光下相似。日光在785nm处发射2mW的光，与黑暗环境相比，APU值略有变化，且更倾向于较低值。卤素灯在785nm处发射6mW的光能，使得基于颜色的表示无法解读。
卤素灯和日光有可能混淆皮肤损伤LDI的结果，而LED和CFL照明则不会。在伤口成像前，应减少任何潜在的日光来源，并完全覆盖或关闭卤素灯。
