Pavithra Dhayalan, Gautam Majumdar, Rama Ranganathan, Swaminathan Rajaraman, Gopal Gopisetty, Ramakrishnan Ayyalur Seshadri, Rajkumar Thangarajan
Department of Molecular Oncology, Cancer Institute (WIA), Adyar, Chennai, 600036, India.
Department of Radiotherapy and Oncology, Regional Cancer Center, Agartala, Tripura, 799006, India.
Cancer Epidemiol. 2018 Apr;53:21-26. doi: 10.1016/j.canep.2018.01.004. Epub 2018 Jan 30.
Gastric cancer is worldwide the third major cause of cancer related death. Risk factors for gastric cancer includes Helicobacter pylori infection, gastric ulcer, less hygienic condition, use of tobacco, alcohol consumption, use of salted, smoked food, genetic alterations etc. In order to identify the risk factors associated with gastric cancer in South Indian population a case-control study involving 200 proven gastric cancer cases and 400 controls was conducted.
A structured questionnaire was used to interview all the subjects who participated in our study. Genotyping assay was performed using Taqman allelic discrimination assay for 5 Single Nucleotide Polymorphisms (SNPs)-TGFβ C-509T, TGFβ T869C, XRCC1 Arg194Trp, IkBα C642T and IL4C-590T.
Odds Ratios (ORs) and 95% confidence intervals (CIs) were calculated using conditional logistic regression. Statistical analysis on socio-economic factors, lifestyle factors had showed that subjects from low socio economic status, use of tobacco and consumption of non-vegetarian food had increased risk of developing gastric cancer. Multi-factorial analysis for the SNPs adjusting for the risk factors obtained in this study showed that TGFΒ C-509T TT genotypes had four fold increased risk of gastric cancer (OR = 4.11, CI = 1.02-16.56) and TGFβ T869C CC genotype had a decreased risk of gastric cancer (OR = 0.21, CI = 0.05-0.85).
Economic status, tobacco use and food habits play a significant role in gastric cancer development. TT genotype for TGFβ C-509T had an increased risk and CC genotype for TGFβ T869C had a decreased risk of gastric cancer in south Indian population after adjusting for socio-economic factors and lifestyle factors.
在全球范围内，胃癌是癌症相关死亡的第三大主要原因。胃癌的风险因素包括幽门螺杆菌感染、胃溃疡、卫生条件较差、吸烟、饮酒、食用腌制和烟熏食品、基因改变等。为了确定印度南部人群中与胃癌相关的风险因素，开展了一项病例对照研究，涉及200例经证实的胃癌病例和400例对照。
使用结构化问卷对参与本研究的所有受试者进行访谈。采用Taqman等位基因鉴别分析法对5个单核苷酸多态性（SNP）——转化生长因子β（TGFβ）C-509T、TGFβ T869C、X线修复交叉互补蛋白1（XRCC1）Arg194Trp、核因子κB抑制蛋白α（IkBα）C642T和白细胞介素4（IL4）C-590T进行基因分型检测。
采用条件逻辑回归计算比值比（OR）和95%置信区间（CI）。对社会经济因素、生活方式因素的统计分析表明，社会经济地位低、吸烟和食用非素食的受试者患胃癌的风险增加。对本研究中获得的风险因素进行校正后的SNP多因素分析表明，TGFΒ C-509T的TT基因型患胃癌的风险增加了四倍（OR = 4.11，CI = 1.02 - 16.56），而TGFβ T869C的CC基因型患胃癌的风险降低（OR = 0.21，CI = 0.05 - 0.85）。
经济状况、吸烟和饮食习惯在胃癌发生中起重要作用。在调整社会经济因素和生活方式因素后，印度南部人群中TGFβ C-509T的TT基因型患胃癌的风险增加，而TGFβ T869C的CC基因型患胃癌的风险降低。
