南非开普敦空气中嗜热型真菌病原体暗色丝孢霉的分子检测。

Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada.

Global Health Institute, University of Antwerp, Antwerp, Belgium.

PLoS Negl Trop Dis. 2018 Jan 22;12(1):e0006174. doi: 10.1371/journal.pntd.0006174. eCollection 2018 Jan.

Emergomyces africanus is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5 Es. africanus propagules per day. Samples were collected in Cape Town, South Africa over 50 weeks by a Burkard spore trap with an alternate orifice. We detected Es. africanus in air samples from 34 days (10%) distributed over 11 weeks. These results suggest environmental exposure to airborne Es. africanus propagules occurs more commonly in endemic areas than previously appreciated.

棘状外瓶霉是一种温度双相真菌，会导致南非免疫功能低下人群的系统性真菌感染。感染被认为是吸入空气中的孢子所致。我们开发了一种定量 PCR 方案，能够每天检测到低至 5 个棘状外瓶霉孢子。在南非开普敦，通过带有交替孔的 Burkard 孢子捕捉器，于 50 周内采集样本。我们在空气样本中检测到棘状外瓶霉，分布在 11 周内的 34 天（10%）。这些结果表明，在流行地区，环境中暴露于空气中的棘状外瓶霉孢子比以前认为的更为常见。