Mattila Sari, Ruotsalainen Pilvi, Jalasvuori Matti
Department of Biological and Environmental Science, Centre of Excellence in Biological Interactions, University of Jyväskylä Jyväskylä Finland.
Front Microbiol. 2015 Nov 13;6:1271. doi: 10.3389/fmicb.2015.01271. eCollection 2015.
Bacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. In this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. We attempted to enrich infectious bacteriophages from sewage against nosocomial drug-resistant bacterial strains of different medically important species in order to evaluate the probability of discovering novel therapeutic phages. Stability and host-range were determined for the acquired phages. Our results suggest that on-demand isolation of phages is possible against Pseudomonas aeruginosa, Salmonella and extended spectrum beta-lactamase Escherichia coli and Klebsiella pneumoniae. The probability of finding suitable phages was less than 40% against vancomycin resistant Enterococcus and Acinetobacter baumannii strains. Furthermore, isolation of new phages against methicillin resistant Staphylococcus aureus strains was found to be very difficult.
噬菌体是细菌病毒，甚至能够杀死多重耐药细菌细胞。因此，噬菌体的治疗用途被认为是传统抗生素的一种可能替代方案。然而，与广谱抗生素相比，噬菌体具有很强的宿主特异性，因此预先制备针对病原体的噬菌体鸡尾酒可能很困难。在本研究中，我们评估了是否有可能从环境库中按需分离噬菌体。我们试图从污水中富集针对不同医学重要物种的医院耐药菌株的感染性噬菌体，以评估发现新型治疗性噬菌体的可能性。对获得的噬菌体进行了稳定性和宿主范围的测定。我们的结果表明，针对铜绿假单胞菌、沙门氏菌以及产超广谱β-内酰胺酶的大肠杆菌和肺炎克雷伯菌，按需分离噬菌体是可能的。针对耐万古霉素肠球菌和鲍曼不动杆菌菌株找到合适噬菌体的概率小于40%。此外，发现针对耐甲氧西林金黄色葡萄球菌菌株分离新噬菌体非常困难。
