Zhu Bojin, Yan Yongjin, Shao Boyun, Tian Luwen, Zhou Weihua
1 Department of Respiratory Medicine, the People's Hospital of Hai'an, Hai'an, Jiangsu, China.
2 Department of Cardiology, the People's Hospital of Hai'an, Hai'an, Jiangsu, China.
J Int Med Res. 2018 Apr;46(4):1517-1527. doi: 10.1177/0300060517750298. Epub 2018 Jan 24.
Objective To explore the prognostic significance of nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylase sirtuin-6 (SIRT6), encoded by the sirtuin 6 ( SIRT6) gene, in a population of Chinese Han patients with non-small cell lung cancer (NSCLC). Methods Cancer tissues and normal lung tissues (>5 cm adjacent to cancer tissue) were collected from Chinese Han patients with NSCLC. Expression levels of SIRT6 and histone H3-acetyl K56 ( H3K56), in cancer and normal lung tissues from patients with NSCLC, were detected by reverse-transcription polymerase chain reaction, Western blot and immunohistochemistry. Correlations between SIRT6 expression and various clinicopathologic features were investigated. Results Out of 86 patients included in the study, mRNA and protein SIRT6 levels were down-regulated in NSCLC tissue versus normal lung tissue, and SIRT6 levels were inversely correlated with H3K56 levels. Positive rates of SIRT6 were significantly correlated with degree of cell differentiation, TNM stage, lymph node metastasis, overall survival and metastasis-free survival. Conclusion Downregulation of SIRT6 expression may promote NSCLC malignancy in the Chinese Han population. SIRT6 may be a potential therapeutic target in Chinese Han patients with NSCLC.
目的 探讨烟酰胺腺嘌呤二核苷酸（NAD）依赖性蛋白脱乙酰酶沉默调节蛋白6（SIRT6）（由沉默调节蛋白6（SIRT6）基因编码）在中国汉族非小细胞肺癌（NSCLC）患者群体中的预后意义。方法 收集中国汉族NSCLC患者的癌组织及正常肺组织（距癌组织>5 cm）。采用逆转录聚合酶链反应、蛋白质免疫印迹法和免疫组织化学法检测NSCLC患者癌组织和正常肺组织中SIRT6及组蛋白H3-乙酰化赖氨酸56（H3K56）的表达水平。研究SIRT6表达与各种临床病理特征之间的相关性。结果 本研究纳入的86例患者中，NSCLC组织中SIRT6的mRNA和蛋白水平相较于正常肺组织下调，且SIRT6水平与H3K56水平呈负相关。SIRT6的阳性率与细胞分化程度、TNM分期、淋巴结转移、总生存期和无转移生存期显著相关。结论 SIRT6表达下调可能促进中国汉族人群NSCLC的恶性进展。SIRT6可能是中国汉族NSCLC患者潜在的治疗靶点。
