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HPLC-PDA 联合化学计量学定量分析苍耳子生品及炮制品的活性成分及质量评价

HPLC-PDA Combined with Chemometrics for Quantitation of Active Components and Quality Assessment of Raw and Processed Fruits of Xanthium strumarium L.

机构信息

Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.

School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 528458, China.

出版信息

Molecules. 2018 Jan 25;23(2):243. doi: 10.3390/molecules23020243.

DOI:10.3390/molecules23020243
PMID:29370133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6017294/
Abstract

As a valuable herbal medicine, the fruits of L. (Xanthii Fructus) have been widely used in raw and processed forms to achieve different therapeutic effects in practice. In this study, a comprehensive strategy was proposed for evaluating the active components in 30 batches of raw and processed Xanthii Fructus (RXF and PXF) samples, based on high-performance liquid chromatography coupled with photodiode array detection (HPLC-PDA). Twelve common peaks were detected and eight compounds of caffeoylquinic acids were simultaneously quantified in RXF and PXF. All the analytes were detected with satisfactory linearity (R² > 0.9991) over wide concentration ranges. Simultaneously, the chemically latent information was revealed by hierarchical cluster analysis (HCA) and principal component analysis (PCA). The results suggest that there were significant differences between RXF and PXF from different regions in terms of the content of eight caffeoylquinic acids. Potential chemical markers for XF were found during processing by chemometrics.

摘要

作为一种有价值的草药，[Xanthii Fructus] 的果实被广泛应用于生品和炮制品中，以在实践中达到不同的治疗效果。在这项研究中，提出了一种基于高效液相色谱-光电二极管阵列检测（HPLC-PDA）的综合策略，用于评估 30 批生品和炮制品[Xanthii Fructus]（RXF 和 PXF）样品中的活性成分。在 RXF 和 PXF 中检测到 12 个常见峰，并同时定量了 8 种咖啡酰奎宁酸类化合物。所有分析物在宽浓度范围内均表现出良好的线性关系（R²＞0.9991）。同时，通过层次聚类分析（HCA）和主成分分析（PCA）揭示了化学潜在信息。结果表明，来自不同地区的 RXF 和 PXF 在 8 种咖啡酰奎宁酸的含量上存在显著差异。通过化学计量学发现了炮制过程中的潜在化学标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/58ac5e7622d0/molecules-23-00243-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/800c7b0503bc/molecules-23-00243-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/079addd9855a/molecules-23-00243-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/da2bf2c5cbb1/molecules-23-00243-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/53d19389bbaa/molecules-23-00243-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/cf2ddfd15318/molecules-23-00243-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/58ac5e7622d0/molecules-23-00243-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/800c7b0503bc/molecules-23-00243-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/0af377745946/molecules-23-00243-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/079addd9855a/molecules-23-00243-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/da2bf2c5cbb1/molecules-23-00243-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/53d19389bbaa/molecules-23-00243-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/cf2ddfd15318/molecules-23-00243-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b1/6017294/58ac5e7622d0/molecules-23-00243-g007.jpg

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