Department of Physics, School of Sciences and Humanities, Nazarbayev University, Astana 010000, Kazakhstan.
School of Engineering and Digital Sciences, Nazarbayev University, Astana 010000, Kazakhstan.
Biosensors (Basel). 2022 Dec 22;13(1):8. doi: 10.3390/bios13010008.
We report non-contact laser-based Brillouin light-scattering (BLS) spectroscopy measurements of the viscoelastic properties of hyperthermally radiofrequency (RF)-heated and ablated bovine liver and chicken flesh tissues with embedded gold nanoparticles (AuNPs). The spatial lateral profile of the local surface temperature in the flesh samples during their hyperthermia was measured through optical backscattering reflectometry (OBR) using Mg−silica-NP-doped sensing fibers distributed with an RF applicator and correlated with viscoelastic variations in heat-affected and ablated tissues. Substantial changes in the tissue stiffness after heating and ablation were directly related to their heat-induced structural modifications. The main proteins responsible for muscle elasticity were denatured and irreversibly aggregated during the RF ablation. At T > 100 °C, the proteins constituting the flesh further shrank and became disorganized, leading to substantial plastic deformation of biotissues. Their uniform destruction with larger thermal lesions and a more viscoelastic network was attained via AuNP-mediated RF hyperthermal ablation. The results demonstrated here pave the way for simultaneous real-time hybrid optical sensing of viscoelasticity and local temperature in biotissues during their denaturation and gelation during hyperthermia for future applications that involve mechanical- and thermal-property-controlled theranostics.
我们报告了基于非接触式激光的布里渊光散射（BLS）光谱测量技术，用于测量射频（RF）加热和烧蚀的牛肝和鸡肉组织的粘弹性特性，这些组织中嵌入了金纳米颗粒（AuNPs）。通过使用 Mg-SiO2-NP 掺杂的传感光纤进行的光学背向散射反射测量（OBR），测量了肉样在高温过程中的局部表面温度的空间横向轮廓，该光纤与 RF 应用器一起分布，并与受热和烧蚀组织中的粘弹性变化相关联。加热和烧蚀后组织硬度的显著变化与它们的热诱导结构改性直接相关。负责肌肉弹性的主要蛋白质在 RF 烧蚀过程中变性并不可逆地聚集。在 T > 100°C 时，构成肉体的蛋白质进一步收缩并变得无序，导致生物组织发生大量塑性变形。通过 AuNP 介导的 RF 超热烧蚀，可以实现具有更大热损伤和更粘弹性网络的均匀破坏。这里的结果为在热疗过程中生物组织的变性和胶凝过程中的粘弹性和局部温度的实时混合光学传感铺平了道路，这为涉及机械和热性能控制的治疗学的未来应用提供了可能。
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