Razmyar J, Jamshidi A, Khanzadi S, Kalidari Gh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Iran J Vet Res. 2017 Fall;18(4):271-274.
This study was designed to evaluate the occurrence of in both broiler chicken farms and packed chicken parts sold at market places in Mashhad, the second most popular Islamic pilgrimage city after Mecca in northeastern Iran. The fresh faecal samples were obtained from broiler farms, while the chicken packs were purchased from retail outlets across the city at market places and samples were obtained from the necks, thighs, and wings. The selective culture was used for isolation of . Out of 40 pooled fresh faecal and 65 packed chicken samples, 14 (35%) and 10 (15.3%) samples were positive, respectively. Some of the isolates from chickens packs (7 out of 10, 70%) and faecal samples (5 out of 14, 36%) were detected as toxigenic (A, B and binary toxins) using molecular identification. From 14 isolates of the faecal samples, five isolates were tcdA and tcdB positive, and none was binary toxin positive. The results of the present study suggest that broiler chickens are a potential source of , which may infect humans through contact or consumption of chicken meat, although the significance of food contamination is entirely unclear, the role of poultry products as a potential source of the infection should be investigated.
本研究旨在评估伊朗东北部仅次于麦加的第二大伊斯兰朝圣城市马什哈德的肉鸡养殖场以及市场上销售的包装鸡肉部位中[某种细菌名称未给出]的出现情况。新鲜粪便样本取自肉鸡养殖场，而鸡肉包装则从全市各地的零售市场购买，并从颈部、大腿和翅膀部位采集样本。采用选择性培养法分离[某种细菌名称未给出]。在40份合并的新鲜粪便样本和65份包装鸡肉样本中，分别有14份（35%）和10份（15.3%）样本呈阳性。使用分子鉴定法检测发现，鸡肉包装样本中的一些分离株（10份中的7份，70%）和粪便样本中的一些分离株（14份中的5份，36%）具有产毒性（A、B和二元毒素）。在14份粪便样本分离株中，5份分离株tcdA和tcdB呈阳性，无一例二元毒素呈阳性。本研究结果表明，肉鸡是[某种细菌名称未给出]的潜在来源，可能通过接触或食用鸡肉感染人类，尽管食品污染的影响尚不完全清楚，但应调查家禽产品作为感染潜在来源的作用。
