一种研究人类口腔黏膜病变中上皮-间充质相互作用的方法。

A method for studying epithelial-mesenchymal interactions in human oral mucosal lesions.

作者信息

Mackenzie I C, Dabelsteen E, Roed-Petersen B

出版信息

Scand J Dent Res. 1979 Jun;87(3):234-43. doi: 10.1111/j.1600-0722.1979.tb00677.x.

DOI:10.1111/j.1600-0722.1979.tb00677.x

Abstract

A system has been developed for examining connective tissue influence on keratinization of normal and leukoplakic human oral epithelia. Paired biopsy specimens of normal and leukoplakic mucosa were left intact or were incubated in 1 mM EDTA to permit epithelial-connective tissue separation and various recombinations of the epithelium and connective tissue. The tissues were transplanting to subdermal sites of "nude" (nu/nu) mice and were protected by polyethylene capsules. 44% of implants performed were recovered apparently vital. Preexisting structure was retained after epithelial-connective tissue separation and self-recombination. Cross-recombined specimens showed changes in patterns of keratinization including changes of keratinized leukoplakic epithelium to a non-keratinized type.

摘要

已开发出一种系统，用于研究结缔组织对正常和白斑性人类口腔上皮角质化的影响。将正常和白斑性黏膜的配对活检标本保持完整，或在1 mM乙二胺四乙酸（EDTA）中孵育，以使上皮-结缔组织分离，并进行上皮和结缔组织的各种重组。将这些组织移植到“裸”（nu/nu）小鼠的皮下部位，并用聚乙烯胶囊进行保护。所进行的植入物中有44%在回收时显然存活。上皮-结缔组织分离和自我重组后，原有结构得以保留。交叉重组的标本显示角质化模式发生了变化，包括角化的白斑上皮转变为非角化类型。