环孢素A对原发性胆汁性肝硬化中免疫球蛋白产生及伴刀豆球蛋白A诱导抑制的体外作用
In vitro effect of cyclosporin A on immunoglobulin production and concanavalin A induced suppression in primary biliary cirrhosis.
作者信息
Al-Aghbar M N, Alexander G J, Nouri-Aria K T, Neuberger J, Eddleston A L, Williams R
出版信息
Gut. 1986 Mar;27(3):317-23. doi: 10.1136/gut.27.3.317.
The in vitro effect of Cyclosporin A on the regulation of immunoglobulin production was investigated in 16 patients with primary biliary cirrhosis. A significant improvement in concanavalin A induced suppression of IgG and IgM producing cells was observed after prior incubation of mononuclear cells with 300 ng/ml Cyclosporin A for 30 minutes. No effect was seen on spontaneous or pokeweed mitogen induced immunoglobulin production, nor on con A induced suppression if Cyclosporin A was added after 24 hours. Incubation of mononuclear cells with a variable dose of Cyclosporin A showed an effect only at 250-500 ng/ml. Higher and lower doses had no effect. This dose dependent effect of Cyclosporin A is likely to be related to a differential inhibitory effect on T helper and T suppressor cells and may underlie the clinical benefit being observed in current clinical trials.
在16例原发性胆汁性肝硬化患者中研究了环孢素A对免疫球蛋白产生调节的体外作用。在用300 ng/ml环孢素A将单核细胞预先孵育30分钟后，观察到伴刀豆球蛋白A诱导的IgG和IgM产生细胞的抑制有显著改善。如果在24小时后添加环孢素A，则对自发或商陆有丝分裂原诱导的免疫球蛋白产生没有影响，对伴刀豆球蛋白A诱导的抑制也没有影响。用不同剂量的环孢素A孵育单核细胞仅在250 - 500 ng/ml时显示出作用。更高和更低的剂量没有作用。环孢素A的这种剂量依赖性作用可能与对辅助性T细胞和抑制性T细胞的不同抑制作用有关，并且可能是当前临床试验中观察到的临床益处的基础。
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