Al-Aghbar M N, Alexander G J, Neuberger J, Nouri-Aria K T, Eddleston A L, Williams R
Clin Exp Immunol. 1986 Mar;63(3):663-70.
Immunoglobulin production and its regulation have been investigated in 36 untreated patients with primary biliary cirrhosis and 14 healthy controls. Using a haemolytic plaque assay, the number of cells spontaneously producing IgG or IgM was normal, while in contrast, proliferation of both IgG and IgM producing cells in the presence of pokeweed mitogen was impaired (P less than 0.01). There was in addition a defect of Con A induced suppression of proliferation of IgG and IgM producing cells (P less than 0.01), which was more marked in those with early disease than in those with advanced disease. In vitro incubation of mononuclear cells with prednisolone, 5 X 10(-8) M, corrected the defect of Con A induced suppression, but had no effect on spontaneous or pokeweed mitogen stimulated immunoglobulin production. Prednisolone was only effective if added prior to exposure to Con A. There is anecdotal evidence that corticosteroids may be beneficial in primary biliary cirrhosis and the results of the present study study suggest that further investigation of their role is warranted.
对36例未经治疗的原发性胆汁性肝硬化患者和14名健康对照者的免疫球蛋白产生及其调节进行了研究。采用溶血空斑试验，自发产生IgG或IgM的细胞数量正常，而相反，在商陆有丝分裂原存在的情况下，产生IgG和IgM的细胞增殖受损（P<0.01）。此外，存在Con A诱导的产生IgG和IgM的细胞增殖抑制缺陷（P<0.01），早期疾病患者比晚期疾病患者更明显。用5×10⁻⁸M泼尼松龙对单核细胞进行体外孵育，纠正了Con A诱导的抑制缺陷，但对自发或商陆有丝分裂原刺激的免疫球蛋白产生没有影响。泼尼松龙只有在接触Con A之前添加才有效。有轶事证据表明皮质类固醇可能对原发性胆汁性肝硬化有益，本研究结果表明有必要进一步研究它们的作用。
