Rosman Raihana, Saifullah Bullo, Maniam Sandra, Dorniani Dena, Hussein Mohd Zobir, Fakurazi Sharida
Institute of Biosciences, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Institute of Advanced Technology (ITMA), Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Nanomaterials (Basel). 2018 Feb 2;8(2):83. doi: 10.3390/nano8020083.
Lung cancer, breast cancer and colorectal cancer are the most prevalent fatal types of cancers globally. Gallic acid (3,4,5-trihydroxybenzoic acid) is a bioactive compound found in plants and foods, such as white tea, witch hazel and it has been reported to possess anticancer, antioxidant and anti-inflammatory properties. In this study we have redesigned our previously reported anticancer nanocomposite formulation with improved drug loading based on iron oxide magnetite nanoparticles coated with polyethylene glycol and loaded with anticancer drug gallic acid (Fe₃O₄-PEG-GA). The in vitro release profile and percentage drug loading were found to be better than our previously reported formulation. The anticancer activity of pure gallic acid (GA), empty carrier (Fe₃O₄-PEG) nanocarrier and of anticancer nanocomposite (Fe₃O₄-PEG-GA) were screened against human lung cancer cells (A549), human breast cancer cells (MCF-7), human colon cancer cells (HT-29) and normal fibroblast cells (3T3) after incubation of 24, 48 and 72 h using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT assay. The designed formulation (Fe₃O₄-PEG-GA) showed better anticancer activity than free gallic acid (GA). The results of the in vitro studies are highly encouraging to conduct the in vivo studies.
肺癌、乳腺癌和结直肠癌是全球最常见的致命癌症类型。没食子酸（3,4,5-三羟基苯甲酸）是一种存在于植物和食物中的生物活性化合物，如白茶、金缕梅，据报道具有抗癌、抗氧化和抗炎特性。在本研究中，我们基于聚乙二醇包覆并负载抗癌药物没食子酸的四氧化三铁磁性纳米颗粒，重新设计了我们之前报道的具有更高载药量的抗癌纳米复合制剂（Fe₃O₄-PEG-GA）。发现其体外释放曲线和载药百分比优于我们之前报道的制剂。使用（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）MTT 法，在孵育 24、48 和 72 小时后，对纯没食子酸（GA）、空载体（Fe₃O₄-PEG）纳米载体和抗癌纳米复合材料（Fe₃O₄-PEG-GA）针对人肺癌细胞（A549）、人乳腺癌细胞（MCF-7）、人结肠癌细胞（HT-29）和正常成纤维细胞（3T3）进行了抗癌活性筛选。所设计的制剂（Fe₃O₄-PEG-GA）显示出比游离没食子酸（GA）更好的抗癌活性。体外研究结果非常鼓舞人心，促使开展体内研究。
