Veryser Lieselotte, Taevernier Lien, Wynendaele Evelien, Verheust Yannick, Dumoulin Ann, De Spiegeleer Bart
Drug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium.
LIWET, Department of Industrial Biological Sciences, Ghent University Campus Kortrijk, Graaf Karel de Goedelaan 5, B-8500 Kortrijk, Belgium.
J Pharm Anal. 2017 Feb;7(1):34-47. doi: 10.1016/j.jpha.2016.09.005. Epub 2016 Sep 24.
and are both plants belonging to the Asteracea family and are traditionally used for their medicinal properties. It has already been shown that some -alkylamides (NAAs) are responsible for these pharmacological actions. Therefore, in the present study, the NAA content of the two plants was analytically characterised. Different extracts were prepared from the roots, the leaves, the stems and the flowers. The structures of NAAs have been assigned in ethanolic extracts of and using high performance liquid chromatography - electrospray ionisation - mass spectrometry (HPLC-ESI-MS) and gas chromatography - electron impact - mass spectrometry (GC-EI-MS). Using both analytical techniques, the structures of 14 and 15 NAAs have been assigned in and , respectively. Structures of two new NAAs, previously never observed in , were assigned: deca-2E,6Z,8E-trienoic acid 2-methylbutylamide (homospilanthol) or a related isomeric compound and deca-2E,4E-dienoic acid -methyl isobutylamide. The structure of homospilanthol or a related isomeric compound was also assigned in for the first time.
[植物名称1]和[植物名称2]均为菊科植物，传统上因其药用特性而被使用。已经表明，一些N-烷基酰胺（NAAs）是这些药理作用的原因。因此，在本研究中，对这两种植物的NAA含量进行了分析表征。从根、叶、茎和花中制备了不同的提取物。使用高效液相色谱-电喷雾电离-质谱（HPLC-ESI-MS）和气相色谱-电子轰击-质谱（GC-EI-MS）在[植物名称1]和[植物名称2]的乙醇提取物中确定了NAAs的结构。使用这两种分析技术，在[植物名称1]和[植物名称2]中分别确定了14种和15种NAAs的结构。确定了两种新的NAAs的结构，这两种结构以前在[植物名称1]中从未观察到：癸-2E,6Z,8E-三烯酸2-甲基丁酰胺（高圣草素）或一种相关的异构化合物以及癸-2E,4E-二烯酸N-甲基异丁酰胺。高圣草素或一种相关的异构化合物的结构也首次在[植物名称2]中被确定。
