Shumkova Rositsa, Georgieva Ani, Radoslavov Georgi, Sirakova Daniela, Dzhebir Gyulnas, Neov Boyko, Bouga Maria, Hristov Peter
Agricultural and Stockbreeding Experimental Station, Agricultural Academy, Smolyan, Bulgaria.
Department of Pathology, Institute of Experimental Morphology, Pathology and Morphology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.
PeerJ. 2018 Jan 31;6:e4252. doi: 10.7717/peerj.4252. eCollection 2018.
and are the two main microsporidian parasites causing nosematosis in the honey bee . The aim of the present study is to investigate the presence of and in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with alone and co-infection were not detected in any samples. These findings suggest that is the dominant species in the Bulgarian honey bee. It is not known when the introduction of in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian . In conclusion, this is the first report for molecular detection of infection of honey bee in Bulgaria. The results showed that is the main species in Bulgaria.
和是导致蜜蜂微孢子虫病的两种主要微孢子虫寄生虫。本研究的目的是调查保加利亚地区和的存在情况。选择16S（SSU）rDNA基因区域进行分析。对来自该国三个不同地区（保加利亚南部、北部和西部）的108个蜜蜂样本进行了双重PCR检测。结果表明，该国北部地区的样本中的患病率最高（77.2%），而山区（保加利亚南部的罗多彼山脉）的样本患病率最低（13.9%）。在任何样本中均未检测到单独感染或共同感染的情况。这些发现表明是保加利亚蜜蜂中的优势物种。尚不清楚在保加利亚是何时引入的，但与世界其他地区一样，该物种已成为保加利亚的优势物种。总之，这是保加利亚蜜蜂感染分子检测的首份报告。结果表明是保加利亚的主要物种。
