五聚酮-ansamycin 微ansamycins A-I 来自 Micromonospora sp. 揭示了不同的聚酮合酶后修饰。
Pentaketide Ansamycin Microansamycins A-I from Micromonospora sp. Reveal Diverse Post-PKS Modifications.
机构信息
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University , Jinan, Shandong 250012, China.
State Key Laboratory of Microbial Technology, Shandong University , Jinan, Shandong 250100, China.
出版信息
Org Lett. 2018 Feb 16;20(4):1058-1061. doi: 10.1021/acs.orglett.7b04018. Epub 2018 Feb 7.
Overexpression of the pathway-specific positive regulator gene mas13 activated the cryptic gene cluster mas, resulting in the isolation of nine novel pentaketide ansamycins, namely, microansamycins A-I (1-9). These results not only revealed a biosynthetic gene cluster of pentaketide ansamycins for the first time but also presented an unprecedented scenario of diverse post-PKS modifications in ansamycin biosynthesis.
通路特异性正调控基因 mas13 的过表达激活了隐秘基因簇 mas，从而分离到了 9 个新型五聚酮安莎霉素，即 microansamycins A-I（1-9）。这些结果不仅首次揭示了五聚酮安莎霉素的生物合成基因簇，而且呈现了安莎霉素生物合成中前所未有的多样的 PKS 后修饰情景。
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