Key Laboratory of Major Crop Diseases, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, China.
Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):3174-3179. doi: 10.1073/pnas.1705927115. Epub 2018 Feb 5.
Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against and pv with no major penalty on key agronomic traits. Map-based cloning reveals that encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple () genes and promotes their turnover. Loss of function of the gene leads to accumulation of mRNAs in the mutant. Furthermore, overexpression of in wild-type rice TP309 confers resistance to , supporting the role of Our discovery of the allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.
携带广谱抗性基因的作物为控制传染病提供了一种有效的策略，因为这些基因通常对病原体的多个菌株甚至多个病原体物种具有抗性。尽管它们很重要，但仅报道了少数几种作物广谱抗性基因。在这里，我们报告了广谱抗性 Kitaake-1 突变体的鉴定和特征，该突变体对 和 pv 具有广谱抗性，对关键农艺性状没有明显的不利影响。基于图谱的克隆表明， 编码一个含有四肽重复（TPRs）的蛋白，它与多个 （）基因的 mRNA 结合，并促进其周转。 的功能丧失导致突变体中 的 mRNA 积累。此外，在野生型水稻 TP309 中过表达 赋予对 的抗性，支持 的作用。我们发现的 等位基因是一个重大的概念性进展，并为培育广谱抗性水稻提供了一个有价值的工具。
