Miao Rui-Xue, Zhu Xin-Xin, Wan Chao-Min, Wang Zhi-Ling, Wen Yang, Li Yi-Yuan
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Exp Ther Med. 2018 Jan;15(1):1081-1086. doi: 10.3892/etm.2017.5461. Epub 2017 Nov 7.
The objective of the present study was to examine whether supplementation has a role in the regulation of the intestinal flora and the development of the immune system of neonatal mice. A total of 30 pregnant BALB/c mice, including their offspring, were randomly divided into three groups: In the maternal intervention group (Ba), maternal mice were treated with from birth until weaning at postnatal day 21 (PD21) followed by administration of saline to the offspring at PD21-28; in the offspring intervention group (Ab), breast-feeding maternal mice were supplemented with saline and offspring were directly supplemented with from PD21-28; in the both maternal and offspring intervention group (Bb), both maternal mice and offspring were supplemented with at PD 0-21 and at PD21-28. While mice in the control group were given the same volume of normal saline. Stool samples from the offspring were collected at PD14, -21 and -28 to observe the intestinal flora by colony counts of spp., spp., spp. and spp. Detection of intestinal secreted immunoglobulin A (sIgA) levels and serum cytokine (interferon-γ, and interleukin-12, -4 and -10) levels in offspring was performed to evaluate the effect on their immune system. The results revealed that compared with the control group, offspring in the Ba group displayed significantly decreased stool colony counts of spp. (t=3.123, P<0.01) at PD14 and significantly decreased counts of spp. at PD14 and -21 (t=2.563, P<0.05 and t=2.292, P<0.05, respectively). Compared with the control group, the stool colony counts of spp. and spp. were significantly increased in the Ba group at PD21 (t=3.085, P<0.01 and t=2.8508, P<0.05, respectively). The Ab group had significantly higher stool colony counts of spp. and spp. at PD28, compared with the control group (Q=7.679, P<0.01 and Q=6.149, P<0.01, respectively). There were no significant differences identified in the sIgA levels of the intestinal fluid and serum cytokine levels between the control group and the intervention groups. In conclusion, administered to breast-feeding maternal mice was able to regulate the intestinal flora balance in their offspring. However, due to insignificant effects on sIgA level and the associated cytokines, had a limited influence on the balance of type 1 vs. type 2 T-helper cells. However, using as an invention may be a safe method for improving the balance of intestinal flora and associated processes in offspring.
本研究的目的是检验补充剂是否在新生小鼠肠道菌群调节及免疫系统发育中发挥作用。总共30只怀孕的BALB/c小鼠及其后代被随机分为三组：在母体干预组（Ba）中，母鼠从出生至出生后第21天（PD21）断奶期间接受[具体物质未提及]处理，随后在PD21 - 28期间给其后代注射生理盐水；在后代干预组（Ab）中，哺乳母鼠补充生理盐水，而后代在PD21 - 28期间直接补充[具体物质未提及]；在母体和后代均干预组（Bb）中，母鼠和后代在PD 0 - 21以及PD21 - 28期间均补充[具体物质未提及]。而对照组小鼠给予相同体积的生理盐水。在PD14、 - 21和 - 28收集后代的粪便样本，通过对[具体菌种未提及]菌属、[具体菌种未提及]菌属、[具体菌种未提及]菌属和[具体菌种未提及]菌属进行菌落计数来观察肠道菌群。检测后代肠道分泌型免疫球蛋白A（sIgA）水平以及血清细胞因子（干扰素 - γ、白细胞介素 - 12、 - 4和 - 10）水平，以评估对其免疫系统的影响。结果显示，与对照组相比：Ba组后代在PD14时[具体菌种未提及]菌属的粪便菌落计数显著降低（t = 3.123，P < 0.01），在PD14和 - 21时[具体菌种未提及]菌属的计数显著降低（分别为t = 2.563，P < 0.05和t = 2.292，P < 0.05）。与对照组相比，Ba组在PD21时[具体菌种未提及]菌属和[具体菌种未提及]菌属的粪便菌落计数显著增加（分别为t = 3.085，P < 0.01和t = 2.8508，P < 0.05）。与对照组相比，Ab组在PD28时[具体菌种未提及]菌属和[具体菌种未提及]菌属的粪便菌落计数显著更高（分别为Q = 7.679，P < 0.01和Q = 6.149，P < 0.01）。对照组与干预组之间在肠液sIgA水平和血清细胞因子水平上未发现显著差异。总之，给哺乳母鼠施用[具体物质未提及]能够调节其后代的肠道菌群平衡。然而，由于对sIgA水平和相关细胞因子影响不显著，[具体物质未提及]对1型与2型辅助性T细胞平衡的影响有限。不过，使用[具体物质未提及]作为一种干预手段可能是改善后代肠道菌群平衡及相关过程的一种安全方法。
