Seynhaeve Ann L B, Ten Hagen Timo L M
Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC.
Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Erasmus MC;
J Vis Exp. 2018 Jan 19(131):55115. doi: 10.3791/55115.
Tumor and tumor vessel development, as well as tumor response to therapeutics, are highly dynamic biological processes. Histology provides static information and is often not sufficient for a correct interpretation. Intravital evaluation, in which a process is followed in time, provides extra and often unexpected information. With the creation of transgenic animals expressing cell-specific markers and live cell tracers, improvements to imaging equipment, and the development of several imaging chambers, intravital microscopy has become an important tool to better understand biological processes. This paper describes an experimental design for the investigation of tumor vessel development and of therapeutic effects in a spatial and temporal manner. Using this setup, the stage of vessel development, tip cell and lumen formation, blood flow, extravasation, an established vascular bed, and vascular destruction can be visualized and followed. Furthermore, therapeutic effects, intratumoral fate, and the localization of chemotherapeutic compounds can also be followed.
肿瘤及肿瘤血管的发育，以及肿瘤对治疗的反应，都是高度动态的生物学过程。组织学提供的是静态信息，往往不足以进行正确解读。活体评估可实时追踪某个过程，能提供额外且常常出人意料的信息。随着表达细胞特异性标志物的转基因动物、活细胞示踪剂的出现，成像设备的改进，以及多种成像室的研发，活体显微镜检查已成为更好理解生物学过程的一项重要工具。本文描述了一种用于以空间和时间方式研究肿瘤血管发育及治疗效果的实验设计。利用该装置，可以可视化并追踪血管发育阶段、尖端细胞和管腔形成、血流、外渗、已形成的血管床以及血管破坏情况。此外，还可以追踪治疗效果、肿瘤内的转归以及化疗化合物的定位。
