Bader Antoine, Cockroft Scott L
EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh, EH9 3FJ, UK.
Chemistry. 2018 Apr 3;24(19):4820-4824. doi: 10.1002/chem.201800756. Epub 2018 Mar 7.
A fundamental principle of digital computer operation is Boolean logic, where inputs and outputs are described by binary integer voltages. Similarly, inputs and outputs may be processed on the molecular level as exemplified by synthetic circuits that exploit the programmability of DNA base-pairing. Unlike modern computers, which execute large numbers of logic gates in parallel, most implementations of molecular logic have been limited to single computing tasks, or sensing applications. This work reports three G-quadruplex-based logic gates that operate simultaneously in a single reaction vessel. The gates respond to unique Boolean DNA inputs by undergoing topological conversion from duplex to G-quadruplex states that were resolved using a thioflavin T dye and gel electrophoresis. The modular, addressable, and label-free approach could be incorporated into DNA-based sensors, or used for resolving and debugging parallel processes in DNA computing applications.
数字计算机操作的一个基本原理是布尔逻辑，其中输入和输出由二进制整数电压来描述。类似地，输入和输出也可以在分子水平上进行处理，利用DNA碱基配对可编程性的合成电路就是例证。与现代计算机并行执行大量逻辑门不同，大多数分子逻辑实现仅限于单个计算任务或传感应用。这项工作报告了三种基于G-四链体的逻辑门，它们在单个反应容器中同时运行。这些逻辑门通过从双链体到G-四链体状态的拓扑转换来响应独特的布尔DNA输入，该转换使用硫黄素T染料和凝胶电泳进行解析。这种模块化、可寻址且无标记的方法可纳入基于DNA的传感器中，或用于解析和调试DNA计算应用中的并行过程。
