Biomass Engineering Research Division, Center for Sustainable Resource Science, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Biomass Engineering Research Division, Center for Sustainable Resource Science, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
Curr Opin Biotechnol. 2018 Dec;54:41-44. doi: 10.1016/j.copbio.2018.01.021. Epub 2018 Feb 20.
Artificial design of metabolic pathways is essential for the production of useful compounds using microbes. Based on this design, heterogeneous genes are introduced into the host, and then various analysis and evaluation methods are conducted to ensure that the target enzyme reactions are functionalized within the cell. In this chapter, we list successful examples of useful compounds produced by designing artificial metabolic pathways, and describe the methods involved in analyzing, evaluating, and optimizing the target enzyme reaction.
人工设计代谢途径对于利用微生物生产有用化合物至关重要。在此设计基础上，将异源基因引入宿主，然后进行各种分析和评估方法，以确保目标酶反应在细胞内具有功能性。在本章中，我们列出了通过设计人工代谢途径生产有用化合物的成功实例，并描述了分析、评估和优化目标酶反应的相关方法。
