Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, 88040-970, Brazil; Departamento de Farmácia, Centro Universitário Barriga Verde, Orleans, 88870-000 Brazil.
Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, 88040-970, Brazil; Departamento de Química, Pontificia Universidad Javeriana, Carrera 7 No.43-82, Bogotá, Colombia.
Biomed Pharmacother. 2018 Apr;100:388-393. doi: 10.1016/j.biopha.2018.02.002. Epub 2018 Feb 16.
The aim of this study was to investigate the sedative activity of the aqueous leaf extract of Passiflora quadrangularis, a species that is widely cultivated and consumed in South America, and to identify its main constituents and elucidate the involvement of the GABAergic pathway in its mechanism of action. The bioguided fractionation of the crude extract showed a positive relationship between the sedative activity of the extract and its flavonoids. The methods employed to identify and isolate its main flavonoids resulted in the identification of vitexin-2''-O-xyloside, vitexin-2''-O-glucoside, orientin-2''-O-xyloside and orientin-2''-O-glucoside. Vitexin-2"-O-xyloside, the major flavonoid of the extract, showed sedative activity after oral administration in mice.
本研究旨在探讨广泛种植和食用于南美洲的西番莲（Passiflora quadrangularis）叶的水提物的镇静活性，并鉴定其主要成分，阐明其作用机制是否涉及 GABA 能途径。粗提物的生物导向分离显示，提取物的镇静活性与其类黄酮之间呈正相关。用于鉴定和分离其主要类黄酮的方法鉴定出了牡荆素-2''-O-木糖苷、牡荆素-2''-O-葡萄糖苷、荭草素-2''-O-木糖苷和荭草素-2''-O-葡萄糖苷。提取物中的主要类黄酮牡荆素-2″-O-木糖苷在口服给予小鼠后显示出镇静活性。
