Department of Analytical Chemistry, Medical University of Bialystok, Poland.
Mass Spectrometry Center, QOPNA, Department of Chemistry, University of Aveiro, Portugal.
J Dermatol Sci. 2018 Jun;90(3):241-252. doi: 10.1016/j.jdermsci.2018.02.002. Epub 2018 Feb 15.
Rutin, due to its polyphenolic structure, has antioxidant properties and can be used as a cytoprotective compound against UV-induced effects on skin cells.
The aim of this study was to examine the effect of rutin on proteomic profile in human skin fibroblasts irradiated with UV dose that induces apoptosis.
Proteome analysis based on the results obtained by the QExactive OrbiTrap mass spectrometer.
Results show that rutin treatment more strongly protects against UVA-induced rather than UVB-induced increases in the total expression of proteins involved in antioxidant (such as SOD, TrxR, and Prxs 1/2) and inflammatory response (e.g., IL-17F, PAK2, and YWHAZ). However, in the case of UVB-irradiated cells, rutin additionally enhances the levels of disulfide-isomerase - an enzyme that is responsible for the formation and breakage of disulfide bonds. Moreover, UVB radiation promotes rutin-Keap1 adduct formation, which leads to the activation of Nrf2, a factor that is responsible for the synthesis of cytoprotective proteins. Furthermore, rutin partially prevents UV-induced apoptosis by restoring the physiological levels of p53, cytochrome c, and cell cycle and apoptosis regulator protein 2 that were increased following irradiation.
In conclusion, our results show that rutin effectively prevents UV-induced damages associated with proinflammatory and prooxidative activity and protects cells against apoptosis.
芦丁由于其多酚结构，具有抗氧化特性，可用作针对皮肤细胞中 UV 诱导效应的细胞保护化合物。
本研究旨在研究芦丁对经 UV 剂量照射诱导凋亡的人皮肤成纤维细胞蛋白质组谱的影响。
基于 QExactive OrbiTrap 质谱仪获得的结果进行蛋白质组分析。
结果表明，芦丁处理对 UVA 诱导而非 UVB 诱导的抗氧化（如 SOD、TrxR 和 Prxs 1/2）和炎症反应（如 IL-17F、PAK2 和 YWHAZ）相关蛋白总表达的增加具有更强的保护作用。然而，在 UVB 照射的细胞中，芦丁还增强了二硫异构酶的水平-一种负责形成和断裂二硫键的酶。此外，UVB 辐射促进了芦丁-Keap1 加合物的形成，从而激活了 Nrf2，该因子负责合成细胞保护蛋白。此外，芦丁通过恢复照射后增加的 p53、细胞色素 c 和细胞周期和凋亡调节剂蛋白 2 的生理水平，部分防止了 UV 诱导的细胞凋亡。
总之，我们的结果表明，芦丁可有效预防与促炎和促氧化活性相关的 UV 诱导损伤，并保护细胞免受凋亡。
