Photoprotective Effects of a Hyperoside-Enriched Fraction Prepared from Thunb. on Ultraviolet B-Induced Skin Aging in Human Fibroblasts through the MAPK Signaling Pathway.

Ultraviolet-B (UVB) irradiation causes skin damage via deleterious effects including oxidative stress, inflammation, and collagen degradation. The photoprotective effects of a hyperoside-enriched fraction obtained from Thunb. () on the attenuation of UVB-induced skin aging in human fibroblasts were investigated. The solvent-partition technique was used to establish the hyperoside-enriched fraction of (HcEA). The active compounds identified in the extracts were hyperoside, quercitrin, chlorogenic acid, and rutin. With regard to the photoprotective effects of on UVB-irradiated dermal fibroblasts, HcEA and hyperoside inhibited intracellular ROS production and inflammatory cytokine secretions (IL-6 and IL-8), while increasing collagen type I synthesis along with downregulating MMP-1 gene and protein expressions. Mechanistically, the hyperoside-enriched fraction obtained from inhibited UVB-irradiated skin aging through regulation of the MAPK signaling pathway by attenuating the activation of JNK/ERK/c-Jun in human dermal fibroblasts. The hyperoside-enriched fraction of exerted potent anti-skin aging properties against UVB exposure. The findings of this study can be applied in the cosmetics industry, as extract can potentially be used in pharmaceutical or cosmetic formulations as a photoprotective or anti-skin aging agent.