Gęgotek Agnieszka, Łuczaj Wojciech, Skrzydlewska Elżbieta
Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland.
Antioxidants (Basel). 2021 Apr 8;10(4):578. doi: 10.3390/antiox10040578.
Ultraviolet (UV) radiation is one of the primary factors responsible for disturbances in human skin cells phospholipid metabolism. Natural compounds that are commonly used to protect skin, due to their lipophilic or hydrophilic nature, show only a narrow range of cytoprotective activity, which prompts research on their combined application. Therefore, the aim of this study was to examine the effect of ascorbic acid and rutin on the phospholipid and ceramide profiles in UV-irradiated fibroblasts cultured in a three-dimensional system that approximates the culture conditions to the dermis. An ultra-high-performance liquid chromatograph coupled with a quadrupole time-of-flight mass spectrometer was used for phospholipid and ceramide profiling. As a result of UVA and UVB cells irradiation, upregulation of phosphatidylcholines, ceramides, and downregulation of sphingomyelins were observed, while treatment with ascorbic acid and rutin of UVA/UVB-irradiated fibroblast promoted these changes to provide cells a stronger response to stress. Moreover, an upregulation of phosphatidylserines in cells exposed to UVB and treated with both antioxidants suggests the stimulation of UV-damaged cells apoptosis. Our findings provide new insight into action of rutin and ascorbic acid on regulation of phospholipid metabolism, which improves dermis fibroblast membrane properties.
紫外线（UV）辐射是导致人类皮肤细胞磷脂代谢紊乱的主要因素之一。由于其亲脂性或亲水性，常用于保护皮肤的天然化合物仅表现出狭窄范围的细胞保护活性，这促使人们对其联合应用进行研究。因此，本研究的目的是在三维系统中培养的紫外线照射的成纤维细胞中，研究抗坏血酸和芦丁对磷脂和神经酰胺谱的影响，该三维系统将培养条件近似于真皮。使用超高效液相色谱仪与四极杆飞行时间质谱仪联用进行磷脂和神经酰胺分析。在UVA和UVB照射细胞后，观察到磷脂酰胆碱、神经酰胺上调，鞘磷脂下调，而用抗坏血酸和芦丁处理UVA/UVB照射的成纤维细胞促进了这些变化，使细胞对应激产生更强的反应。此外，在暴露于UVB并用两种抗氧化剂处理的细胞中，磷脂酰丝氨酸上调表明刺激了紫外线损伤细胞的凋亡。我们的研究结果为芦丁和抗坏血酸对磷脂代谢调节的作用提供了新的见解，这改善了真皮成纤维细胞膜的特性。
