Uwamahoro Marie Christine, Massicotte Richard, Hurtubise Yves, Gagné-Bourque François, Mafu Akier Assanta, Yahia L'Hocine
Laboratory of Innovation and Analysis of Bioperformance, Ecole Polytechnique de Montreal, Montreal, QC, Canada.
Centre Intégré de Santé et de Services Sociaux de Lanaudière, Joliette, QC, Canada.
Front Public Health. 2018 Feb 5;6:18. doi: 10.3389/fpubh.2018.00018. eCollection 2018.
Spore-forming pathogenic bacteria, such as , are associated with nosocomial infection, leading to the increased use of sporicidal disinfectants, which impacts socioeconomic costs. However, can be prevented using microorganisms such as , a prophylactic agent that has been proven to be effective against it in recent tests or it can be controlled by sporicidal disinfectants. These disinfectants against spores should be evaluated according to a known and recommended standard. Unfortunately, some newly manufactured disinfectants like Bioxy products have not yet been tested. ASTM E2197-11 is a standard test that uses stainless steel disks (1 cm in diameter) as carriers, and the performance of the test formulation is calculated by comparing the number of viable test organisms to that on the control carriers. Surface tests are preferable for evaluating disinfectants with sporicidal effects on hard surfaces. This study applies improved methods, based on the ASTM E2197-11 standard, for evaluating and comparing the sporicidal efficacies of several disinfectants against spores of and , which are used as the test organisms. With the improved method, all spores were recovered through vortexing and membrane filtration. The results show that chlorine-based products are effective in 5 min and Bioxy products at 5% w/v are effective in 10 min. Although Bioxy products may take longer to prove their effectiveness, their non-harmful effects to hospital surfaces and people have been well established in the literature.
形成芽孢的致病细菌，如 ，与医院感染相关，导致杀孢子消毒剂的使用增加，这影响了社会经济成本。然而， 可以使用微生物如 来预防， 是一种预防性制剂，在最近的测试中已被证明对其有效，或者可以通过杀孢子消毒剂来控制。这些针对孢子的消毒剂应根据已知且推荐的标准进行评估。不幸的是，一些新生产的消毒剂，如Bioxy产品，尚未经过测试。ASTM E2197 - 11是一种标准测试，使用不锈钢圆盘（直径1厘米）作为载体，通过将存活测试微生物的数量与对照载体上的数量进行比较来计算测试制剂的性能。表面测试对于评估对硬表面有杀孢子作用的消毒剂更为可取。本研究基于ASTM E2197 - 11标准应用改进方法，以评估和比较几种消毒剂对用作测试生物的 和 的孢子的杀孢子效果。采用改进方法，通过涡旋和膜过滤回收了所有孢子。结果表明，氯基产品在5分钟内有效，5% w/v的Bioxy产品在10分钟内有效。尽管Bioxy产品可能需要更长时间来证明其有效性，但其对医院表面和人员的无害作用在文献中已有充分记载。
