Graduate Program in Agricultural Sciences, Universidade Estadual da Paraíba (UEPB), Campina Grande/PB 58429-500, Brazil.
Department of Biology, Universidade Estadual da Paraíba (UEPB).
Molecules. 2018 Feb 17;23(2):448. doi: 10.3390/molecules23020448.
Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology.
来自未培养微生物的碱性植酸酶可以将植酸水解为低磷酸化肌醇和无机磷酸盐，具有作为农业工业添加剂的巨大潜力。宏基因组学的发展源于一个不可避免的事实，即迄今尚未培养的微生物代表了地球上大多数环境中绝大多数的生物。在这项研究中，使用宏基因组学策略从红稻残渣和蓖麻饼中克隆了一个编码植酸酶的基因。该基因与最近发表的最相似基因的氨基酸同一性低于 60%。该植酸酶被命名为 PhyRC001，并进行了生化特性分析。这种重组蛋白对植酸钠表现出活性，表明 PhyRC001 是一种水解酶。该酶的最适 pH 值为 7.0，最适温度为 35°C。β-螺旋桨植酸酶具有作为饲料添加剂的巨大潜力，因为它们是唯一在中性 pH 值下具有高活性的植酸酶。因此，探索和利用β-螺旋桨植酸酶功能的潜在机制可能对生物技术有很大的益处。
