Amadi Victor A, Hariharan Harry, Arya Gitanjali, Matthew-Belmar Vanessa, Nicholas-Thomas Roxanne, Pinckney Rhonda, Sharma Ravindra, Johnson Roger
Pathobiology DepartmentSchool of Veterinary MedicineSt. George's UniversitySt. George'sGrenadaWest Indies.
Office International des Epizooties (OIE) Salmonella Reference LaboratoryPublic Health Agency of CanadaNational Microbiology at GuelphGuelphOntarioCanada.
Vet Med Sci. 2017 Nov 9;4(1):26-34. doi: 10.1002/vms3.84. eCollection 2018 Feb.
Non-typhoidal salmonellosis remains an important public health problem worldwide. Dogs may harbour in their intestines and can easily shed in the environment with the possibility of transmission to humans. Thus, monitoring is essential to understand the role of dogs in zoonotic transmission. The objectives of this study were to determine the shedding of by owned, apparently healthy dogs in Grenada, West Indies, to identify the serovars, and to examine their antimicrobial susceptibility profiles. Faecal samples collected during August to October, 2016 from 144 non-diarrhoeic owned dogs were examined by enrichment and selective culture for the presence of spp. Eight (5.6%) of the tested animals were culture positive, yielding 35 isolates that belonged to six serovars of subspecies . These were serovars Arechavaleta from two dogs, Arechavaleta and Montevideo from one dog, and Javiana, Rubislaw, Braenderup and Kiambu from one dog each. All these serovars have been reported as causes of human salmonellosis globally. Antimicrobial susceptibility tests on 35 isolates showed absence of resistance to the currently used drugs for cases of human salmonellosis, including ciprofloxacin and cefotaxime. One isolate (2.9%) was resistant to neomycin, two isolates (5.7%) showed intermediate susceptibility to neomycin, and another (2.9%) had intermediate susceptibility to tetracycline. This is the first report of isolation and antimicrobial susceptibilities of non-typhoidal serovars from dogs in Grenada. This study shows that dogs in Grenada may be involved in the epidemiology of salmonellosis.
非伤寒沙门氏菌病仍然是全球一个重要的公共卫生问题。狗的肠道中可能携带（非伤寒沙门氏菌），并且能轻易地在环境中散播，存在传播给人类的可能性。因此，监测对于了解狗在人畜共患病传播中的作用至关重要。本研究的目的是确定西印度群岛格林纳达拥有的、看似健康的狗的（非伤寒沙门氏菌）排泄情况，鉴定血清型，并检测它们的抗菌药敏谱。2016年8月至10月期间从144只无腹泻的家养狗采集粪便样本，通过增菌和选择性培养检测非伤寒沙门氏菌属细菌的存在情况。8只（5.6%）受试动物培养呈阳性，获得35株非伤寒沙门氏菌分离株，它们属于非伤寒沙门氏菌亚种的六个血清型。其中两只狗的血清型为阿雷查瓦莱塔，一只狗的血清型为阿雷查瓦莱塔和蒙得维的亚，另外四只狗的血清型分别为哈维亚纳、鲁比斯劳、布伦德鲁普和坎布。所有这些血清型在全球范围内均被报道为人类沙门氏菌病的病因。对35株分离株进行的抗菌药敏试验表明，它们对目前用于治疗人类沙门氏菌病的药物（包括环丙沙星和头孢噻肟）均无耐药性。一株分离株（2.9%）对新霉素耐药，两株分离株（5.7%）对新霉素表现出中介敏感性，另一株（2.9%）对四环素表现出中介敏感性。这是格林纳达狗身上非伤寒沙门氏菌血清型分离及抗菌药敏情况的首次报告。本研究表明，格林纳达的狗可能参与了沙门氏菌病的流行病学传播。
