Klein Mathias, Islam Zia-Ul, Knudsen Peter Boldsen, Carrillo Martina, Swinnen Steve, Workman Mhairi, Nevoigt Elke
Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany.
Department of Systems Biology, Building 223, Søltofts Plads, Technical University of Denmark, Lyngby 2800 Kgs, Denmark.
Metab Eng Commun. 2016 Sep 29;3:252-257. doi: 10.1016/j.meteno.2016.09.001. eCollection 2016 Dec.
Glycerol is an abundant by-product during biodiesel production and additionally has several assets compared to sugars when used as a carbon source for growing microorganisms in the context of biotechnological applications. However, most strains of the platform production organism grow poorly in synthetic glycerol medium. It has been hypothesized that the uptake of glycerol could be a major bottleneck for the utilization of glycerol in . This species exclusively relies on an active transport system for glycerol uptake. This work demonstrates that the expression of predicted glycerol facilitators (Fps1 homologues) from superior glycerol-utilizing yeast species such as , , and significantly improves the growth performance on glycerol of the previously selected glycerol-consuming wild-type strain (CBS 6412-13A). The maximum specific growth rate increased from 0.13 up to 0.18 h and a biomass yield coefficient of 0.56 g/g was observed. These results pave the way for exploiting the assets of glycerol in the production of fuels, chemicals and pharmaceuticals based on baker's yeast.
甘油是生物柴油生产过程中一种丰富的副产物，并且在生物技术应用中用作微生物生长的碳源时，与糖类相比还具有若干优势。然而，大多数平台生产生物体菌株在合成甘油培养基中生长不佳。据推测，甘油的摄取可能是甘油利用的主要瓶颈。该物种完全依赖主动运输系统来摄取甘油。这项研究表明，来自优良甘油利用酵母物种（如、、和）的预测甘油促进剂（Fps1同源物）的表达显著提高了先前选定的甘油消耗野生型菌株（CBS 6412 - 13A）在甘油上的生长性能。最大比生长速率从0.13提高到了0.18 h，并且观察到生物量产率系数为0.56 g/g。这些结果为在基于面包酵母的燃料、化学品和药品生产中利用甘油的优势铺平了道路。
