Liu Jian, Qiu Xuemei, Wang Daoming, Li Yantao, Zong Yang, Liu Yichen, Zhang Yue, Yang Ping, Zuo Yong, Yang Huanming, Wang Jian, Du Yutao, Zi Jin
1 213636 BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China.
2 213636 BGI -Shenzhen, Shenzhen 518083, China.
J Int Med Res. 2018 Apr;46(4):1414-1427. doi: 10.1177/0300060517752733. Epub 2018 Feb 22.
Objective This study was performed to investigate the effects of age and sex on 10 salivary steroid hormones and analyze the correlations between salivary and plasma hormones. Methods The concentrations of 10 salivary steroid hormones in 1090 Chinese adult volunteers were examined using liquid chromatography-tandem mass spectrometry, and a related investigation was performed on the concentrations of salivary hormones in this population. Results The concentrations of androstenedione (A4), 17α-hydroxyprogesterone (17-OHP), aldosterone (ALD), cortisone (COR), corticosterone (CORT), cortisol (F), progesterone (P), and testosterone were significantly different between men and women (Student's t-test). Differences in 17-OHP and ALD concentrations were highly significant between women in the follicular and luteal phases of their menstrual cycle (Student's t-test). Five salivary steroid hormones (17-OHP, A4, CORT, COR, and F) significantly decreased with increasing age (Kruskal-Wallis test). A high linear correlation between salivary and plasma 17-OHP, P, A4, and F were observed with obvious sex-related differences (Pearson's correlation, r > 0.7). Conclusions Our results provide important knowledge regarding the descriptive characteristics of salivary hormones in relation to age and sex and their correlations with plasma hormones.
目的 本研究旨在探讨年龄和性别对10种唾液类固醇激素的影响，并分析唾液激素与血浆激素之间的相关性。方法 采用液相色谱-串联质谱法检测1090名中国成年志愿者唾液中10种类固醇激素的浓度，并对该人群的唾液激素浓度进行相关调查。结果 雄烯二酮（A4）、17α-羟孕酮（17-OHP）、醛固酮（ALD）、可的松（COR）、皮质酮（CORT）、皮质醇（F）、孕酮（P）和睾酮在男性和女性之间的浓度存在显著差异（Student's t检验）。在月经周期的卵泡期和黄体期，女性的17-OHP和ALD浓度差异非常显著（Student's t检验）。随着年龄的增长，5种唾液类固醇激素（17-OHP、A4、CORT、COR和F）显著下降（Kruskal-Wallis检验）。观察到唾液和血浆中的17-OHP、P、A4和F之间存在高度线性相关性，且存在明显的性别差异（Pearson相关性，r>0.7）。结论 我们的结果提供了关于唾液激素与年龄和性别相关的描述性特征及其与血浆激素相关性的重要知识。
