Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Frontier Institute of Science and Technology, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, MI 48109, USA; Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
J Colloid Interface Sci. 2018 May 15;518:252-262. doi: 10.1016/j.jcis.2018.02.036. Epub 2018 Feb 13.
Controlling cellular alignment and elongation has been demonstrated as an important parameter for developing nerve tissue engineering scaffolds. Many approaches have been developed to guide cellular orientation for nerve regeneration such as micropatterning techniques. However, most of materials used for developing micropatterning scaffolds lack of bioactivity and biofunctionability. Here we present a functional conductive micropatterned scaffold based on bioactive conductive biodegradable polyurethane prepared using a micro-molding technique. These conductive micropatterned scaffolds are able to not only induce the Schwann cells (SCs) alignment and elongation by the micropatterned surface but also enhance the nerve growth factor (NGF) gene expression of SCs by the bioactivity of these materials. Additionally, the combined effect of the bioactivity of such conductive materials and the micropatterned structure also dramatically promotes the neurite extension and elongation of PC12 cells in a highly aligned direction. These data suggest that these conductive micropatterned scaffolds that easily control cellular orientation and organization, and dramatically enhance NGF gene expression and significantly induce the neurite extension of PC12 cells, have a great potential for nerve regeneration applications.
控制细胞的排列和伸长已被证明是开发神经组织工程支架的一个重要参数。已经开发了许多方法来引导细胞的方向以促进神经再生，例如微图案化技术。然而，大多数用于开发微图案化支架的材料缺乏生物活性和生物功能性。在这里，我们提出了一种基于生物活性导电可生物降解聚氨酯的功能性导电微图案化支架，该支架是使用微成型技术制备的。这些导电微图案化支架不仅可以通过微图案化表面诱导施万细胞（SCs）的排列和伸长，还可以通过这些材料的生物活性增强SCs 的神经生长因子（NGF）基因表达。此外，这种导电材料的生物活性和微图案结构的组合效应也极大地促进了 PC12 细胞在高度排列方向上的神经突延伸和伸长。这些数据表明，这些导电微图案化支架可以轻松控制细胞的取向和组织，极大地增强 NGF 基因表达，并显著诱导 PC12 细胞的神经突延伸，具有很大的神经再生应用潜力。
